Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1817 and GFF1818 are separated by 9 nucleotides GFF1818 and GFF1819 are separated by 115 nucleotides GFF1819 and GFF1820 overlap by 14 nucleotides GFF1820 and GFF1821 are separated by 6 nucleotides
GFF1817 - Cytochrome c-type protein NapC, at 76 to 678
GFF1817
GFF1818 - Nitrate reductase cytochrome c550-type subunit, at 688 to 1,137
GFF1818
GFF1819 - Polyferredoxin NapH (periplasmic nitrate reductase), at 1,253 to 2,122
GFF1819
GFF1820 - Ferredoxin-type protein NapG (periplasmic nitrate reductase), at 2,109 to 2,804
GFF1820
GFF1821 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 2,811 to 5,297
GFF1821
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.322 kb on - strand, within GFF1817 at 0.467 kb on + strand, within GFF1817 at 0.525 kb on + strand, within GFF1817 at 0.720 kb on + strand at 0.721 kb on - strand at 0.755 kb on - strand, within GFF1818 at 0.808 kb on - strand, within GFF1818 at 0.964 kb on + strand, within GFF1818 at 1.076 kb on - strand, within GFF1818 at 1.166 kb on - strand at 1.227 kb on - strand at 1.231 kb on - strand at 1.444 kb on + strand, within GFF1819 at 1.494 kb on - strand, within GFF1819 at 1.526 kb on - strand, within GFF1819 at 1.625 kb on + strand, within GFF1819 at 1.708 kb on - strand, within GFF1819 at 1.711 kb on + strand, within GFF1819 at 1.712 kb on + strand, within GFF1819 at 1.713 kb on - strand, within GFF1819 at 1.713 kb on - strand, within GFF1819 at 1.713 kb on - strand, within GFF1819 at 1.713 kb on - strand, within GFF1819 at 1.715 kb on - strand, within GFF1819 at 1.791 kb on - strand, within GFF1819 at 1.955 kb on - strand, within GFF1819 at 1.955 kb on - strand, within GFF1819 at 1.963 kb on - strand, within GFF1819 at 1.963 kb on - strand, within GFF1819 at 1.963 kb on - strand, within GFF1819 at 2.043 kb on - strand at 2.161 kb on - strand at 2.197 kb on - strand, within GFF1820 at 2.804 kb on + strand at 2.813 kb on - strand at 2.828 kb on + strand at 2.926 kb on + strand at 2.926 kb on + strand at 2.926 kb on + strand at 2.926 kb on + strand at 2.927 kb on - strand at 2.927 kb on - strand at 2.927 kb on - strand at 2.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 322 - GFF1817 0.41 -0.5 467 + GFF1817 0.65 -0.0 525 + GFF1817 0.74 +0.1 720 + +0.3 721 - +0.1 755 - GFF1818 0.15 +0.4 808 - GFF1818 0.27 +0.4 964 + GFF1818 0.61 +0.1 1,076 - GFF1818 0.86 -0.5 1,166 - +0.1 1,227 - -0.0 1,231 - +0.1 1,444 + GFF1819 0.22 +0.6 1,494 - GFF1819 0.28 -0.1 1,526 - GFF1819 0.31 +0.7 1,625 + GFF1819 0.43 +0.1 1,708 - GFF1819 0.52 -0.2 1,711 + GFF1819 0.53 +0.7 1,712 + GFF1819 0.53 +1.1 1,713 - GFF1819 0.53 -0.0 1,713 - GFF1819 0.53 +0.1 1,713 - GFF1819 0.53 +0.6 1,713 - GFF1819 0.53 +0.6 1,715 - GFF1819 0.53 +0.3 1,791 - GFF1819 0.62 -0.4 1,955 - GFF1819 0.81 +0.3 1,955 - GFF1819 0.81 -0.3 1,963 - GFF1819 0.82 +0.3 1,963 - GFF1819 0.82 -0.2 1,963 - GFF1819 0.82 +0.5 2,043 - +0.0 2,161 - -0.5 2,197 - GFF1820 0.13 -1.9 2,804 + +0.4 2,813 - +0.1 2,828 + -0.4 2,926 + -1.5 2,926 + -0.5 2,926 + -0.3 2,926 + -0.7 2,927 - +0.1 2,927 - -0.4 2,927 - -0.9 2,927 - -0.2
Or see this region's nucleotide sequence