Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1665 and GFF1666 are separated by 153 nucleotides GFF1666 and GFF1667 are separated by 46 nucleotides GFF1667 and GFF1668 are separated by 43 nucleotides GFF1668 and GFF1669 are separated by 20 nucleotides
GFF1665 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 50,157 to 51,023
GFF1665
GFF1666 - Methyl-accepting chemotaxis protein, at 51,177 to 52,838
GFF1666
GFF1667 - hypothetical protein, at 52,885 to 53,031
GFF1667
GFF1668 - Positive regulator of CheA protein activity (CheW), at 53,075 to 53,578
GFF1668
GFF1669 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 53,599 to 55,608
GFF1669
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1 at 50.186 kb on - strand at 50.482 kb on - strand, within GFF1665 at 50.521 kb on + strand, within GFF1665 at 50.526 kb on - strand, within GFF1665 at 50.633 kb on - strand, within GFF1665 at 50.768 kb on + strand, within GFF1665 at 50.920 kb on + strand, within GFF1665 at 50.926 kb on - strand, within GFF1665 at 51.024 kb on - strand at 51.035 kb on - strand at 51.050 kb on - strand at 51.050 kb on - strand at 51.108 kb on - strand at 51.150 kb on + strand at 51.269 kb on + strand at 51.269 kb on + strand at 51.270 kb on - strand at 51.693 kb on + strand, within GFF1666 at 51.693 kb on + strand, within GFF1666 at 51.802 kb on - strand, within GFF1666 at 51.805 kb on - strand, within GFF1666 at 52.070 kb on + strand, within GFF1666 at 52.071 kb on - strand, within GFF1666 at 52.071 kb on - strand, within GFF1666 at 52.336 kb on + strand, within GFF1666 at 52.403 kb on + strand, within GFF1666 at 52.428 kb on - strand, within GFF1666 at 52.776 kb on + strand at 52.839 kb on - strand at 53.030 kb on - strand at 53.274 kb on - strand, within GFF1668 at 53.402 kb on - strand, within GFF1668 at 53.429 kb on - strand, within GFF1668 at 53.513 kb on - strand, within GFF1668 at 53.581 kb on + strand at 53.581 kb on + strand at 53.582 kb on - strand at 53.620 kb on - strand at 53.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 50,186 - +0.2 50,482 - GFF1665 0.37 +0.0 50,521 + GFF1665 0.42 -0.5 50,526 - GFF1665 0.43 -1.1 50,633 - GFF1665 0.55 +0.0 50,768 + GFF1665 0.70 +0.0 50,920 + GFF1665 0.88 -0.4 50,926 - GFF1665 0.89 +0.5 51,024 - -0.8 51,035 - -0.0 51,050 - +0.4 51,050 - -0.3 51,108 - -0.6 51,150 + -0.3 51,269 + -0.7 51,269 + -0.1 51,270 - +0.0 51,693 + GFF1666 0.31 -0.1 51,693 + GFF1666 0.31 +0.7 51,802 - GFF1666 0.38 -2.3 51,805 - GFF1666 0.38 +0.5 52,070 + GFF1666 0.54 +0.5 52,071 - GFF1666 0.54 -0.2 52,071 - GFF1666 0.54 +0.2 52,336 + GFF1666 0.70 +0.6 52,403 + GFF1666 0.74 +0.2 52,428 - GFF1666 0.75 +0.6 52,776 + -0.3 52,839 - +0.5 53,030 - -0.9 53,274 - GFF1668 0.39 -0.2 53,402 - GFF1668 0.65 +0.5 53,429 - GFF1668 0.70 +1.7 53,513 - GFF1668 0.87 +0.2 53,581 + +0.2 53,581 + +0.3 53,582 - +0.6 53,620 - -0.1 53,724 - +0.8
Or see this region's nucleotide sequence