Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1274

Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1272 and GFF1273 overlap by 29 nucleotidesGFF1273 and GFF1274 overlap by 4 nucleotidesGFF1274 and GFF1275 are separated by 235 nucleotidesGFF1275 and GFF1276 are separated by 286 nucleotides GFF1272 - Phage holin, at 1,014 to 1,349 GFF1272 GFF1273 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 1,321 to 1,785 GFF1273 GFF1274 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 1,782 to 2,255 GFF1274 GFF1275 - FIG01045439: hypothetical protein, at 2,491 to 2,892 GFF1275 GFF1276 - Terminase small subunit, at 3,179 to 3,724 GFF1276 Position (kb) 1 2 3Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 0.879 kb on + strandat 0.945 kb on + strandat 0.981 kb on - strandat 1.013 kb on + strandat 1.132 kb on + strand, within GFF1272at 1.133 kb on - strand, within GFF1272at 1.134 kb on + strand, within GFF1272at 1.134 kb on + strand, within GFF1272at 1.134 kb on + strand, within GFF1272at 1.195 kb on + strand, within GFF1272at 1.196 kb on - strand, within GFF1272at 1.304 kb on + strand, within GFF1272at 1.476 kb on + strand, within GFF1273at 1.566 kb on + strand, within GFF1273at 1.616 kb on - strand, within GFF1273at 1.638 kb on - strand, within GFF1273at 1.665 kb on - strand, within GFF1273at 1.888 kb on - strand, within GFF1274at 1.899 kb on - strand, within GFF1274at 1.904 kb on + strand, within GFF1274at 2.022 kb on - strand, within GFF1274at 2.221 kb on - strandat 2.284 kb on + strandat 2.339 kb on + strandat 2.369 kb on + strandat 2.547 kb on + strand, within GFF1275at 2.548 kb on - strand, within GFF1275at 2.561 kb on - strand, within GFF1275at 2.574 kb on - strand, within GFF1275at 2.574 kb on - strand, within GFF1275at 2.673 kb on - strand, within GFF1275at 2.673 kb on - strand, within GFF1275at 2.736 kb on + strand, within GFF1275at 2.737 kb on - strand, within GFF1275at 2.738 kb on + strand, within GFF1275at 2.739 kb on - strand, within GFF1275at 2.739 kb on - strand, within GFF1275at 2.743 kb on - strand, within GFF1275at 2.896 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI
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879 + +0.2
945 + +0.4
981 - -1.4
1,013 + -0.5
1,132 + GFF1272 0.35 +1.1
1,133 - GFF1272 0.35 +0.3
1,134 + GFF1272 0.36 +1.3
1,134 + GFF1272 0.36 -0.3
1,134 + GFF1272 0.36 +0.5
1,195 + GFF1272 0.54 -0.3
1,196 - GFF1272 0.54 +0.1
1,304 + GFF1272 0.86 +0.1
1,476 + GFF1273 0.33 +0.0
1,566 + GFF1273 0.53 +0.3
1,616 - GFF1273 0.63 -1.2
1,638 - GFF1273 0.68 -1.0
1,665 - GFF1273 0.74 -0.1
1,888 - GFF1274 0.22 -1.2
1,899 - GFF1274 0.25 -3.7
1,904 + GFF1274 0.26 +0.0
2,022 - GFF1274 0.51 -0.6
2,221 - -0.1
2,284 + +1.1
2,339 + -0.7
2,369 + +1.9
2,547 + GFF1275 0.14 -0.3
2,548 - GFF1275 0.14 +0.1
2,561 - GFF1275 0.17 -0.2
2,574 - GFF1275 0.21 +0.4
2,574 - GFF1275 0.21 -0.3
2,673 - GFF1275 0.45 -0.2
2,673 - GFF1275 0.45 -1.8
2,736 + GFF1275 0.61 -0.4
2,737 - GFF1275 0.61 +0.2
2,738 + GFF1275 0.61 -0.2
2,739 - GFF1275 0.62 -0.3
2,739 - GFF1275 0.62 +0.1
2,743 - GFF1275 0.63 +0.1
2,896 + +0.1

Or see this region's nucleotide sequence