Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1272 and GFF1273 overlap by 29 nucleotides GFF1273 and GFF1274 overlap by 4 nucleotides GFF1274 and GFF1275 are separated by 235 nucleotides GFF1275 and GFF1276 are separated by 286 nucleotides
GFF1272 - Phage holin, at 1,014 to 1,349
GFF1272
GFF1273 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 1,321 to 1,785
GFF1273
GFF1274 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 1,782 to 2,255
GFF1274
GFF1275 - FIG01045439: hypothetical protein, at 2,491 to 2,892
GFF1275
GFF1276 - Terminase small subunit, at 3,179 to 3,724
GFF1276
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 0.879 kb on + strand at 0.945 kb on + strand at 0.981 kb on - strand at 1.013 kb on + strand at 1.132 kb on + strand, within GFF1272 at 1.133 kb on - strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.195 kb on + strand, within GFF1272 at 1.196 kb on - strand, within GFF1272 at 1.304 kb on + strand, within GFF1272 at 1.476 kb on + strand, within GFF1273 at 1.566 kb on + strand, within GFF1273 at 1.616 kb on - strand, within GFF1273 at 1.638 kb on - strand, within GFF1273 at 1.665 kb on - strand, within GFF1273 at 1.888 kb on - strand, within GFF1274 at 1.899 kb on - strand, within GFF1274 at 1.904 kb on + strand, within GFF1274 at 2.022 kb on - strand, within GFF1274 at 2.221 kb on - strand at 2.284 kb on + strand at 2.339 kb on + strand at 2.369 kb on + strand at 2.547 kb on + strand, within GFF1275 at 2.548 kb on - strand, within GFF1275 at 2.561 kb on - strand, within GFF1275 at 2.574 kb on - strand, within GFF1275 at 2.574 kb on - strand, within GFF1275 at 2.673 kb on - strand, within GFF1275 at 2.673 kb on - strand, within GFF1275 at 2.736 kb on + strand, within GFF1275 at 2.737 kb on - strand, within GFF1275 at 2.738 kb on + strand, within GFF1275 at 2.739 kb on - strand, within GFF1275 at 2.739 kb on - strand, within GFF1275 at 2.743 kb on - strand, within GFF1275 at 2.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 879 + +0.2 945 + +0.4 981 - -1.4 1,013 + -0.5 1,132 + GFF1272 0.35 +1.1 1,133 - GFF1272 0.35 +0.3 1,134 + GFF1272 0.36 +1.3 1,134 + GFF1272 0.36 -0.3 1,134 + GFF1272 0.36 +0.5 1,195 + GFF1272 0.54 -0.3 1,196 - GFF1272 0.54 +0.1 1,304 + GFF1272 0.86 +0.1 1,476 + GFF1273 0.33 +0.0 1,566 + GFF1273 0.53 +0.3 1,616 - GFF1273 0.63 -1.2 1,638 - GFF1273 0.68 -1.0 1,665 - GFF1273 0.74 -0.1 1,888 - GFF1274 0.22 -1.2 1,899 - GFF1274 0.25 -3.7 1,904 + GFF1274 0.26 +0.0 2,022 - GFF1274 0.51 -0.6 2,221 - -0.1 2,284 + +1.1 2,339 + -0.7 2,369 + +1.9 2,547 + GFF1275 0.14 -0.3 2,548 - GFF1275 0.14 +0.1 2,561 - GFF1275 0.17 -0.2 2,574 - GFF1275 0.21 +0.4 2,574 - GFF1275 0.21 -0.3 2,673 - GFF1275 0.45 -0.2 2,673 - GFF1275 0.45 -1.8 2,736 + GFF1275 0.61 -0.4 2,737 - GFF1275 0.61 +0.2 2,738 + GFF1275 0.61 -0.2 2,739 - GFF1275 0.62 -0.3 2,739 - GFF1275 0.62 +0.1 2,743 - GFF1275 0.63 +0.1 2,896 + +0.1
Or see this region's nucleotide sequence