Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF104 and GFF105 are separated by 77 nucleotides GFF105 and GFF106 are separated by 201 nucleotides GFF106 and GFF107 are separated by 106 nucleotides
GFF104 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 88,480 to 90,870
GFF104
GFF105 - Blue copper oxidase CueO precursor, at 90,948 to 92,558
GFF105
GFF106 - Predicted chaperone lipoprotein YacC, potentially involved in protein secretion, at 92,760 to 93,107
GFF106
GFF107 - Spermidine synthase (EC 2.5.1.16), at 93,214 to 94,074
GFF107
Position (kb)
90
91
92
93 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 89.988 kb on + strand, within GFF104 at 89.988 kb on + strand, within GFF104 at 89.989 kb on - strand, within GFF104 at 89.989 kb on - strand, within GFF104 at 90.084 kb on - strand, within GFF104 at 90.084 kb on - strand, within GFF104 at 90.225 kb on - strand, within GFF104 at 90.493 kb on - strand, within GFF104 at 90.540 kb on + strand, within GFF104 at 90.540 kb on + strand, within GFF104 at 90.562 kb on - strand, within GFF104 at 90.562 kb on - strand, within GFF104 at 90.673 kb on + strand at 90.673 kb on + strand at 90.673 kb on + strand at 90.673 kb on + strand at 90.688 kb on - strand at 90.725 kb on + strand at 90.751 kb on - strand at 90.757 kb on + strand at 90.778 kb on + strand at 90.778 kb on + strand at 90.779 kb on - strand at 90.832 kb on + strand at 90.833 kb on - strand at 90.844 kb on + strand at 91.012 kb on + strand at 91.012 kb on + strand at 91.012 kb on + strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.277 kb on + strand, within GFF105 at 91.397 kb on + strand, within GFF105 at 91.397 kb on + strand, within GFF105 at 91.398 kb on - strand, within GFF105 at 91.563 kb on - strand, within GFF105 at 91.795 kb on + strand, within GFF105 at 91.796 kb on - strand, within GFF105 at 91.898 kb on - strand, within GFF105 at 91.952 kb on + strand, within GFF105 at 91.953 kb on - strand, within GFF105 at 92.345 kb on + strand, within GFF105 at 92.417 kb on + strand at 92.559 kb on - strand at 92.572 kb on + strand at 92.572 kb on + strand at 92.591 kb on + strand at 92.592 kb on - strand at 92.641 kb on - strand at 92.659 kb on - strand at 92.659 kb on - strand at 92.659 kb on - strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.806 kb on + strand, within GFF106 at 92.807 kb on - strand, within GFF106 at 92.816 kb on + strand, within GFF106 at 92.817 kb on - strand, within GFF106 at 92.821 kb on - strand, within GFF106 at 92.908 kb on + strand, within GFF106 at 92.976 kb on + strand, within GFF106 at 92.976 kb on + strand, within GFF106 at 93.102 kb on - strand at 93.106 kb on - strand at 93.159 kb on + strand at 93.160 kb on - strand at 93.163 kb on + strand at 93.164 kb on - strand at 93.289 kb on + strand at 93.318 kb on - strand, within GFF107 at 93.318 kb on - strand, within GFF107 at 93.371 kb on + strand, within GFF107 at 93.371 kb on + strand, within GFF107 at 93.401 kb on - strand, within GFF107 at 93.488 kb on + strand, within GFF107
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 89,988 + GFF104 0.63 -0.0 89,988 + GFF104 0.63 +0.1 89,989 - GFF104 0.63 -0.0 89,989 - GFF104 0.63 +0.1 90,084 - GFF104 0.67 -0.6 90,084 - GFF104 0.67 -0.1 90,225 - GFF104 0.73 -1.2 90,493 - GFF104 0.84 +0.1 90,540 + GFF104 0.86 -1.0 90,540 + GFF104 0.86 +0.1 90,562 - GFF104 0.87 +0.4 90,562 - GFF104 0.87 +0.1 90,673 + -0.8 90,673 + +0.2 90,673 + +0.3 90,673 + -0.9 90,688 - -0.2 90,725 + -0.8 90,751 - -0.7 90,757 + +0.0 90,778 + +0.0 90,778 + +1.3 90,779 - +0.7 90,832 + -1.2 90,833 - -0.9 90,844 + +0.0 91,012 + -1.3 91,012 + -0.0 91,012 + -0.2 91,013 - -0.1 91,013 - -0.1 91,013 - +0.2 91,013 - -0.2 91,013 - -0.3 91,013 - -0.8 91,013 - +0.2 91,013 - +0.5 91,277 + GFF105 0.20 -0.7 91,397 + GFF105 0.28 -0.2 91,397 + GFF105 0.28 -0.5 91,398 - GFF105 0.28 -0.1 91,563 - GFF105 0.38 +0.7 91,795 + GFF105 0.53 -1.3 91,796 - GFF105 0.53 +0.1 91,898 - GFF105 0.59 +0.5 91,952 + GFF105 0.62 -0.9 91,953 - GFF105 0.62 -0.3 92,345 + GFF105 0.87 -1.4 92,417 + +1.1 92,559 - -0.4 92,572 + +0.4 92,572 + -0.1 92,591 + +0.1 92,592 - -0.2 92,641 - +1.1 92,659 - +0.4 92,659 - -0.4 92,659 - -0.1 92,663 + -0.3 92,663 + -0.1 92,806 + GFF106 0.13 -0.4 92,807 - GFF106 0.14 -0.3 92,816 + GFF106 0.16 -0.1 92,817 - GFF106 0.16 +0.4 92,821 - GFF106 0.18 -0.1 92,908 + GFF106 0.43 +0.1 92,976 + GFF106 0.62 -0.7 92,976 + GFF106 0.62 -0.4 93,102 - +0.2 93,106 - +1.9 93,159 + +0.0 93,160 - -2.3 93,163 + -0.5 93,164 - -0.7 93,289 + +0.7 93,318 - GFF107 0.12 +0.0 93,318 - GFF107 0.12 -0.6 93,371 + GFF107 0.18 -0.7 93,371 + GFF107 0.18 -0.2 93,401 - GFF107 0.22 +0.6 93,488 + GFF107 0.32 -0.0
Or see this region's nucleotide sequence