Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF102 and GFF103 are separated by 57 nucleotides GFF103 and GFF104 are separated by 205 nucleotides GFF104 and GFF105 are separated by 77 nucleotides
GFF102 - Carbonic anhydrase (EC 4.2.1.1), at 87,018 to 87,680
GFF102
GFF103 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 87,738 to 88,274
GFF103
GFF104 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 88,480 to 90,870
GFF104
GFF105 - Blue copper oxidase CueO precursor, at 90,948 to 92,558
GFF105
Position (kb)
88
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1 at 87.663 kb on + strand at 87.679 kb on - strand at 87.686 kb on + strand at 87.690 kb on + strand at 87.690 kb on + strand at 87.691 kb on - strand at 87.691 kb on - strand at 87.693 kb on - strand at 87.701 kb on + strand at 87.701 kb on + strand at 87.769 kb on + strand at 87.770 kb on - strand at 87.897 kb on - strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.956 kb on + strand, within GFF103 at 87.972 kb on - strand, within GFF103 at 87.999 kb on - strand, within GFF103 at 88.137 kb on - strand, within GFF103 at 88.190 kb on - strand, within GFF103 at 88.223 kb on + strand at 88.322 kb on - strand at 88.325 kb on - strand at 88.355 kb on - strand at 88.432 kb on - strand at 88.434 kb on - strand at 88.479 kb on + strand at 88.512 kb on - strand at 88.650 kb on - strand at 88.793 kb on + strand, within GFF104 at 89.349 kb on + strand, within GFF104 at 89.476 kb on + strand, within GFF104 at 89.499 kb on + strand, within GFF104 at 89.589 kb on + strand, within GFF104 at 89.590 kb on - strand, within GFF104 at 89.598 kb on + strand, within GFF104 at 89.668 kb on - strand, within GFF104 at 89.674 kb on - strand, within GFF104 at 89.941 kb on - strand, within GFF104 at 89.944 kb on + strand, within GFF104 at 89.944 kb on + strand, within GFF104 at 89.944 kb on + strand, within GFF104 at 89.988 kb on + strand, within GFF104 at 89.988 kb on + strand, within GFF104 at 89.989 kb on - strand, within GFF104 at 89.989 kb on - strand, within GFF104 at 90.084 kb on - strand, within GFF104 at 90.084 kb on - strand, within GFF104 at 90.225 kb on - strand, within GFF104 at 90.493 kb on - strand, within GFF104 at 90.540 kb on + strand, within GFF104 at 90.540 kb on + strand, within GFF104 at 90.562 kb on - strand, within GFF104 at 90.562 kb on - strand, within GFF104 at 90.673 kb on + strand at 90.673 kb on + strand at 90.673 kb on + strand at 90.673 kb on + strand at 90.688 kb on - strand at 90.725 kb on + strand at 90.751 kb on - strand at 90.757 kb on + strand at 90.778 kb on + strand at 90.778 kb on + strand at 90.779 kb on - strand at 90.832 kb on + strand at 90.833 kb on - strand at 90.844 kb on + strand at 91.012 kb on + strand at 91.012 kb on + strand at 91.012 kb on + strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.013 kb on - strand at 91.277 kb on + strand, within GFF105 at 91.397 kb on + strand, within GFF105 at 91.397 kb on + strand, within GFF105 at 91.398 kb on - strand, within GFF105 at 91.563 kb on - strand, within GFF105 at 91.795 kb on + strand, within GFF105 at 91.796 kb on - strand, within GFF105
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 87,663 + -0.7 87,679 - +0.1 87,686 + -0.4 87,690 + +1.1 87,690 + -1.2 87,691 - +0.1 87,691 - +1.7 87,693 - +0.5 87,701 + +0.1 87,701 + -0.9 87,769 + -0.7 87,770 - -0.6 87,897 - GFF103 0.30 -0.2 87,956 + GFF103 0.41 -0.9 87,956 + GFF103 0.41 -1.2 87,956 + GFF103 0.41 -0.7 87,972 - GFF103 0.44 -1.8 87,999 - GFF103 0.49 -0.1 88,137 - GFF103 0.74 +0.0 88,190 - GFF103 0.84 -0.6 88,223 + -0.5 88,322 - +0.6 88,325 - -0.1 88,355 - -1.6 88,432 - -0.1 88,434 - +0.1 88,479 + +0.3 88,512 - +0.1 88,650 - +0.5 88,793 + GFF104 0.13 -0.6 89,349 + GFF104 0.36 +1.0 89,476 + GFF104 0.42 +1.1 89,499 + GFF104 0.43 -0.9 89,589 + GFF104 0.46 -1.7 89,590 - GFF104 0.46 -0.1 89,598 + GFF104 0.47 +0.5 89,668 - GFF104 0.50 +0.9 89,674 - GFF104 0.50 -0.3 89,941 - GFF104 0.61 -0.2 89,944 + GFF104 0.61 -0.6 89,944 + GFF104 0.61 -0.2 89,944 + GFF104 0.61 -0.5 89,988 + GFF104 0.63 -0.0 89,988 + GFF104 0.63 +0.1 89,989 - GFF104 0.63 -0.0 89,989 - GFF104 0.63 +0.1 90,084 - GFF104 0.67 -0.6 90,084 - GFF104 0.67 -0.1 90,225 - GFF104 0.73 -1.2 90,493 - GFF104 0.84 +0.1 90,540 + GFF104 0.86 -1.0 90,540 + GFF104 0.86 +0.1 90,562 - GFF104 0.87 +0.4 90,562 - GFF104 0.87 +0.1 90,673 + -0.8 90,673 + +0.2 90,673 + +0.3 90,673 + -0.9 90,688 - -0.2 90,725 + -0.8 90,751 - -0.7 90,757 + +0.0 90,778 + +0.0 90,778 + +1.3 90,779 - +0.7 90,832 + -1.2 90,833 - -0.9 90,844 + +0.0 91,012 + -1.3 91,012 + -0.0 91,012 + -0.2 91,013 - -0.1 91,013 - -0.1 91,013 - +0.2 91,013 - -0.2 91,013 - -0.3 91,013 - -0.8 91,013 - +0.2 91,013 - +0.5 91,277 + GFF105 0.20 -0.7 91,397 + GFF105 0.28 -0.2 91,397 + GFF105 0.28 -0.5 91,398 - GFF105 0.28 -0.1 91,563 - GFF105 0.38 +0.7 91,795 + GFF105 0.53 -1.3 91,796 - GFF105 0.53 +0.1
Or see this region's nucleotide sequence