Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2086 and PfGW456L13_2087 are separated by 24 nucleotides PfGW456L13_2087 and PfGW456L13_2088 overlap by 4 nucleotides PfGW456L13_2088 and PfGW456L13_2089 overlap by 1 nucleotides PfGW456L13_2089 and PfGW456L13_2090 overlap by 4 nucleotides
PfGW456L13_2086 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 2,292,270 to 2,293,280
_2086
PfGW456L13_2087 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 2,293,305 to 2,293,490
_2087
PfGW456L13_2088 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 2,293,487 to 2,294,251
_2088
PfGW456L13_2089 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 2,294,251 to 2,294,715
_2089
PfGW456L13_2090 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 2,294,712 to 2,295,731
_2090
Position (kb)
2294
2295 Strain fitness (log2 ratio)
-1
0
1 at 2293.278 kb on + strand at 2294.319 kb on + strand, within PfGW456L13_2089 at 2294.319 kb on + strand, within PfGW456L13_2089 at 2294.491 kb on + strand, within PfGW456L13_2089 at 2294.623 kb on + strand, within PfGW456L13_2089 at 2294.623 kb on + strand, within PfGW456L13_2089
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,293,278 + -0.5 2,294,319 + PfGW456L13_2089 0.15 -0.6 2,294,319 + PfGW456L13_2089 0.15 +0.0 2,294,491 + PfGW456L13_2089 0.52 -0.0 2,294,623 + PfGW456L13_2089 0.80 -0.2 2,294,623 + PfGW456L13_2089 0.80 -0.0
Or see this region's nucleotide sequence