Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF975

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF974 and GFF975 are separated by 275 nucleotidesGFF975 and GFF976 are separated by 2 nucleotidesGFF976 and GFF977 are separated by 181 nucleotidesGFF977 and GFF978 overlap by 4 nucleotides GFF974 - FIG01045697: hypothetical protein, at 160,535 to 161,530 GFF974 GFF975 - FIG074102: hypothetical protein, at 161,806 to 162,717 GFF975 GFF976 - hypothetical protein, at 162,720 to 162,833 GFF976 GFF977 - Putative inner membrane or exported, at 163,015 to 163,383 GFF977 GFF978 - Dienelactone hydrolase family, at 163,380 to 164,054 GFF978 Position (kb) 161 162 163Strain fitness (log2 ratio) -1 0 1at 161.444 kb on - strandat 162.123 kb on + strand, within GFF975at 162.131 kb on + strand, within GFF975at 162.450 kb on - strand, within GFF975at 162.718 kb on + strandat 162.718 kb on + strandat 162.847 kb on - strandat 162.871 kb on + strandat 162.968 kb on + strandat 163.043 kb on - strandat 163.484 kb on + strand, within GFF978at 163.517 kb on - strand, within GFF978at 163.524 kb on - strand, within GFF978at 163.588 kb on + strand, within GFF978at 163.698 kb on + strand, within GFF978

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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161,444 - -0.0
162,123 + GFF975 0.35 -1.1
162,131 + GFF975 0.36 -0.4
162,450 - GFF975 0.71 -0.0
162,718 + +0.1
162,718 + -0.6
162,847 - +0.3
162,871 + -0.1
162,968 + -0.7
163,043 - -1.5
163,484 + GFF978 0.15 +0.9
163,517 - GFF978 0.20 -0.1
163,524 - GFF978 0.21 -0.2
163,588 + GFF978 0.31 +1.2
163,698 + GFF978 0.47 +0.1

Or see this region's nucleotide sequence