Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF850

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF847 and GFF848 are separated by 15 nucleotidesGFF848 and GFF849 are separated by 9 nucleotidesGFF849 and GFF850 are separated by 13 nucleotidesGFF850 and GFF851 are separated by 9 nucleotidesGFF851 and GFF852 overlap by 1 nucleotides GFF847 - Ascorbate-specific PTS system, EIIC component, at 28,582 to 29,979 GFF847 GFF848 - Ascorbate-specific PTS system, EIIB component (EC 2.7.1.69), at 29,995 to 30,300 GFF848 GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774 GFF849 GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438 GFF850 GFF851 - L-ribulose-5-phosphate 3-epimerase UlaE (EC 5.1.3.22) (L-ascorbate utilization protein E), at 31,448 to 32,302 GFF851 GFF852 - L-ribulose-5-phosphate 4-epimerase UlaF (EC 5.1.3.4) (L-ascorbate utilization protein F), at 32,302 to 32,988 GFF852 Position (kb) 30 31 32Strain fitness (log2 ratio) -1 0 1 2at 29.907 kb on + strandat 29.908 kb on - strandat 29.908 kb on - strandat 29.908 kb on - strandat 30.002 kb on + strandat 30.002 kb on + strandat 30.006 kb on + strandat 30.006 kb on + strandat 30.007 kb on - strandat 30.007 kb on - strandat 30.172 kb on - strand, within GFF848at 30.205 kb on + strand, within GFF848at 30.298 kb on + strandat 30.369 kb on + strand, within GFF849at 30.772 kb on + strandat 31.009 kb on + strand, within GFF850at 31.010 kb on - strand, within GFF850at 31.191 kb on - strand, within GFF850at 31.741 kb on + strand, within GFF851at 31.748 kb on - strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.815 kb on - strand, within GFF851at 31.861 kb on - strand, within GFF851at 31.946 kb on - strand, within GFF851at 31.967 kb on + strand, within GFF851at 31.968 kb on - strand, within GFF851at 31.976 kb on - strand, within GFF851at 32.034 kb on + strand, within GFF851at 32.089 kb on + strand, within GFF851at 32.093 kb on - strand, within GFF851at 32.189 kb on + strand, within GFF851at 32.325 kb on - strandat 32.325 kb on - strandat 32.370 kb on + strandat 32.422 kb on - strand, within GFF852

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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29,907 + -0.2
29,908 - +0.3
29,908 - -0.1
29,908 - +1.2
30,002 + +1.3
30,002 + -0.3
30,006 + -0.2
30,006 + +1.1
30,007 - -1.3
30,007 - -1.1
30,172 - GFF848 0.58 -0.7
30,205 + GFF848 0.69 +0.1
30,298 + -0.1
30,369 + GFF849 0.13 +0.4
30,772 + -1.7
31,009 + GFF850 0.34 -0.1
31,010 - GFF850 0.34 -0.6
31,191 - GFF850 0.62 -1.1
31,741 + GFF851 0.34 -0.2
31,748 - GFF851 0.35 -0.3
31,811 + GFF851 0.42 +0.4
31,811 + GFF851 0.42 -0.0
31,815 - GFF851 0.43 -1.1
31,861 - GFF851 0.48 +1.8
31,946 - GFF851 0.58 -0.4
31,967 + GFF851 0.61 -0.3
31,968 - GFF851 0.61 -0.3
31,976 - GFF851 0.62 +0.2
32,034 + GFF851 0.69 +0.2
32,089 + GFF851 0.75 +1.2
32,093 - GFF851 0.75 +0.2
32,189 + GFF851 0.87 +0.1
32,325 - -0.6
32,325 - +0.1
32,370 + +0.3
32,422 - GFF852 0.17 -1.1

Or see this region's nucleotide sequence