Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF847 and GFF848 are separated by 15 nucleotides GFF848 and GFF849 are separated by 9 nucleotides GFF849 and GFF850 are separated by 13 nucleotides GFF850 and GFF851 are separated by 9 nucleotides GFF851 and GFF852 overlap by 1 nucleotides
GFF847 - Ascorbate-specific PTS system, EIIC component, at 28,582 to 29,979
GFF847
GFF848 - Ascorbate-specific PTS system, EIIB component (EC 2.7.1.69), at 29,995 to 30,300
GFF848
GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774
GFF849
GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438
GFF850
GFF851 - L-ribulose-5-phosphate 3-epimerase UlaE (EC 5.1.3.22) (L-ascorbate utilization protein E), at 31,448 to 32,302
GFF851
GFF852 - L-ribulose-5-phosphate 4-epimerase UlaF (EC 5.1.3.4) (L-ascorbate utilization protein F), at 32,302 to 32,988
GFF852
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-1
0
1
2 at 29.907 kb on + strand at 29.908 kb on - strand at 29.908 kb on - strand at 29.908 kb on - strand at 30.002 kb on + strand at 30.002 kb on + strand at 30.006 kb on + strand at 30.006 kb on + strand at 30.007 kb on - strand at 30.007 kb on - strand at 30.172 kb on - strand, within GFF848 at 30.205 kb on + strand, within GFF848 at 30.298 kb on + strand at 30.369 kb on + strand, within GFF849 at 30.772 kb on + strand at 31.009 kb on + strand, within GFF850 at 31.010 kb on - strand, within GFF850 at 31.191 kb on - strand, within GFF850 at 31.741 kb on + strand, within GFF851 at 31.748 kb on - strand, within GFF851 at 31.811 kb on + strand, within GFF851 at 31.811 kb on + strand, within GFF851 at 31.815 kb on - strand, within GFF851 at 31.861 kb on - strand, within GFF851 at 31.946 kb on - strand, within GFF851 at 31.967 kb on + strand, within GFF851 at 31.968 kb on - strand, within GFF851 at 31.976 kb on - strand, within GFF851 at 32.034 kb on + strand, within GFF851 at 32.089 kb on + strand, within GFF851 at 32.093 kb on - strand, within GFF851 at 32.189 kb on + strand, within GFF851 at 32.325 kb on - strand at 32.325 kb on - strand at 32.370 kb on + strand at 32.422 kb on - strand, within GFF852
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 29,907 + -0.2 29,908 - +0.3 29,908 - -0.1 29,908 - +1.2 30,002 + +1.3 30,002 + -0.3 30,006 + -0.2 30,006 + +1.1 30,007 - -1.3 30,007 - -1.1 30,172 - GFF848 0.58 -0.7 30,205 + GFF848 0.69 +0.1 30,298 + -0.1 30,369 + GFF849 0.13 +0.4 30,772 + -1.7 31,009 + GFF850 0.34 -0.1 31,010 - GFF850 0.34 -0.6 31,191 - GFF850 0.62 -1.1 31,741 + GFF851 0.34 -0.2 31,748 - GFF851 0.35 -0.3 31,811 + GFF851 0.42 +0.4 31,811 + GFF851 0.42 -0.0 31,815 - GFF851 0.43 -1.1 31,861 - GFF851 0.48 +1.8 31,946 - GFF851 0.58 -0.4 31,967 + GFF851 0.61 -0.3 31,968 - GFF851 0.61 -0.3 31,976 - GFF851 0.62 +0.2 32,034 + GFF851 0.69 +0.2 32,089 + GFF851 0.75 +1.2 32,093 - GFF851 0.75 +0.2 32,189 + GFF851 0.87 +0.1 32,325 - -0.6 32,325 - +0.1 32,370 + +0.3 32,422 - GFF852 0.17 -1.1
Or see this region's nucleotide sequence