Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF74 and GFF75 are separated by 85 nucleotides GFF75 and GFF76 are separated by 410 nucleotides GFF76 and GFF77 are separated by 160 nucleotides GFF77 and GFF78 are separated by 36 nucleotides
GFF74 - Fimbriae usher protein StfC, at 55,129 to 57,786
GFF74
GFF75 - Major fimbrial subunit StfA, at 57,872 to 58,432
GFF75
GFF76 - hypothetical protein, at 58,843 to 58,968
GFF76
GFF77 - FIG01046082: hypothetical protein, at 59,129 to 59,335
GFF77
GFF78 - Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), permease component FhuB, at 59,372 to 61,429
GFF78
Position (kb) 57
58
59 Strain fitness (log2 ratio) -1
0
1 at 56.899 kb on + strand, within GFF74 at 56.900 kb on - strand, within GFF74 at 56.900 kb on - strand, within GFF74 at 56.973 kb on + strand, within GFF74 at 56.973 kb on + strand, within GFF74 at 57.138 kb on + strand, within GFF74 at 57.209 kb on + strand, within GFF74 at 57.210 kb on - strand, within GFF74 at 57.268 kb on + strand, within GFF74 at 57.455 kb on - strand, within GFF74 at 57.506 kb on + strand, within GFF74 at 57.544 kb on - strand at 57.553 kb on - strand at 57.704 kb on + strand at 57.708 kb on + strand at 57.735 kb on + strand at 57.797 kb on - strand at 57.880 kb on - strand at 58.208 kb on - strand, within GFF75 at 59.387 kb on - strand at 59.406 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 56,899 + GFF74 0.67 +1.3 56,900 - GFF74 0.67 +0.9 56,900 - GFF74 0.67 +0.9 56,973 + GFF74 0.69 -0.5 56,973 + GFF74 0.69 -0.2 57,138 + GFF74 0.76 -0.2 57,209 + GFF74 0.78 -0.1 57,210 - GFF74 0.78 -0.3 57,268 + GFF74 0.80 +0.3 57,455 - GFF74 0.88 -0.2 57,506 + GFF74 0.89 +0.7 57,544 - -0.2 57,553 - -0.5 57,704 + -0.3 57,708 + -0.1 57,735 + +0.5 57,797 - +0.5 57,880 - +0.4 58,208 - GFF75 0.60 -0.7 59,387 - -0.3 59,406 + +0.1
Or see this region's nucleotide sequence