Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF706 and GFF707 are separated by 476 nucleotides GFF707 and GFF708 are separated by 115 nucleotides GFF708 and GFF709 are separated by 28 nucleotides
GFF706 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 63,328 to 64,074
GFF706
GFF707 - Non-specific DNA-binding protein Dps / Iron-binding ferritin-like antioxidant protein / Ferroxidase (EC 1.16.3.1), at 64,551 to 65,054
GFF707
GFF708 - FIG01046955: hypothetical protein, at 65,170 to 65,328
GFF708
GFF709 - Putative DMT superfamily metabolite efflux protein precursor, at 65,357 to 66,244
GFF709
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 63.571 kb on + strand, within GFF706 at 63.581 kb on + strand, within GFF706 at 63.708 kb on + strand, within GFF706 at 63.796 kb on - strand, within GFF706 at 63.801 kb on + strand, within GFF706 at 63.826 kb on - strand, within GFF706 at 63.907 kb on - strand, within GFF706 at 64.039 kb on - strand at 64.060 kb on + strand at 64.121 kb on - strand at 64.121 kb on - strand at 64.195 kb on - strand at 64.259 kb on + strand at 64.260 kb on - strand at 64.365 kb on + strand at 64.428 kb on + strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.430 kb on + strand at 64.430 kb on + strand at 64.431 kb on - strand at 64.433 kb on - strand at 64.440 kb on + strand at 64.440 kb on + strand at 64.440 kb on + strand at 64.531 kb on + strand at 64.540 kb on + strand at 64.541 kb on - strand at 64.547 kb on - strand at 64.718 kb on + strand, within GFF707 at 64.718 kb on + strand, within GFF707 at 64.878 kb on - strand, within GFF707 at 65.035 kb on - strand at 65.062 kb on + strand at 65.408 kb on - strand at 65.578 kb on - strand, within GFF709 at 65.757 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.878 kb on + strand, within GFF709 at 65.979 kb on + strand, within GFF709 at 65.980 kb on - strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.985 kb on - strand, within GFF709 at 65.988 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.016 kb on - strand, within GFF709
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 63,571 + GFF706 0.33 +0.3 63,581 + GFF706 0.34 +0.6 63,708 + GFF706 0.51 -0.4 63,796 - GFF706 0.63 +1.0 63,801 + GFF706 0.63 -2.4 63,826 - GFF706 0.67 +0.0 63,907 - GFF706 0.78 -0.7 64,039 - +0.2 64,060 + +0.1 64,121 - -0.6 64,121 - -0.7 64,195 - +0.1 64,259 + -0.9 64,260 - -1.1 64,365 + +0.4 64,428 + +0.5 64,429 - -0.1 64,429 - +0.8 64,429 - +0.3 64,429 - +0.5 64,430 + +0.6 64,430 + -0.0 64,431 - +0.2 64,433 - +0.1 64,440 + +0.3 64,440 + -0.5 64,440 + +0.3 64,531 + +1.9 64,540 + -0.2 64,541 - +0.1 64,547 - +0.5 64,718 + GFF707 0.33 -0.2 64,718 + GFF707 0.33 -1.0 64,878 - GFF707 0.65 -0.7 65,035 - -0.4 65,062 + +0.0 65,408 - -0.3 65,578 - GFF709 0.25 +0.1 65,757 + GFF709 0.45 +0.7 65,821 + GFF709 0.52 -0.2 65,821 + GFF709 0.52 -0.1 65,822 - GFF709 0.52 -1.1 65,822 - GFF709 0.52 +0.0 65,822 - GFF709 0.52 +0.2 65,822 - GFF709 0.52 -0.3 65,822 - GFF709 0.52 -0.3 65,878 + GFF709 0.59 -0.4 65,979 + GFF709 0.70 +0.0 65,980 - GFF709 0.70 +2.3 65,984 + GFF709 0.71 -0.3 65,984 + GFF709 0.71 -0.1 65,984 + GFF709 0.71 -0.1 65,985 - GFF709 0.71 -0.4 65,988 + GFF709 0.71 -0.2 66,015 + GFF709 0.74 +0.2 66,015 + GFF709 0.74 +0.0 66,015 + GFF709 0.74 -0.1 66,016 - GFF709 0.74 +0.6
Or see this region's nucleotide sequence