Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF678 and GFF679 are separated by 21 nucleotides GFF679 and GFF680 are separated by 2 nucleotides GFF680 and GFF681 overlap by 8 nucleotides GFF681 and GFF682 are separated by 1 nucleotides GFF682 and GFF683 are separated by 47 nucleotides
GFF678 - Molybdenum cofactor biosynthesis protein MoaA, at 37,702 to 38,691
GFF678
GFF679 - Molybdenum cofactor biosynthesis protein MoaB, at 38,713 to 39,225
GFF679
GFF680 - Molybdenum cofactor biosynthesis protein MoaC, at 39,228 to 39,713
GFF680
GFF681 - Molybdenum cofactor biosynthesis protein MoaD, at 39,706 to 39,951
GFF681
GFF682 - Molybdenum cofactor biosynthesis protein MoaE, at 39,953 to 40,405
GFF682
GFF683 - Putative membrane protein, at 40,453 to 41,157
GFF683
Position (kb) 39
40 Strain fitness (log2 ratio) -2
-1
0
1 at 38.322 kb on + strand, within GFF678 at 38.337 kb on - strand, within GFF678 at 38.519 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.582 kb on - strand, within GFF678 at 38.659 kb on - strand at 38.678 kb on - strand at 39.247 kb on + strand at 39.510 kb on - strand, within GFF680 at 39.635 kb on + strand, within GFF680 at 39.640 kb on + strand, within GFF680 at 39.641 kb on - strand, within GFF680 at 39.712 kb on - strand at 39.745 kb on - strand, within GFF681 at 40.403 kb on + strand at 40.403 kb on + strand at 40.510 kb on + strand at 40.510 kb on + strand at 40.510 kb on + strand at 40.511 kb on - strand at 40.526 kb on - strand, within GFF683 at 40.550 kb on + strand, within GFF683 at 40.585 kb on - strand, within GFF683 at 40.687 kb on - strand, within GFF683
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 38,322 + GFF678 0.63 +0.1 38,337 - GFF678 0.64 -1.0 38,519 + GFF678 0.83 +0.8 38,581 + GFF678 0.89 +0.3 38,581 + GFF678 0.89 +0.2 38,581 + GFF678 0.89 -0.1 38,582 - GFF678 0.89 +1.1 38,659 - -0.6 38,678 - +0.7 39,247 + -0.6 39,510 - GFF680 0.58 -2.1 39,635 + GFF680 0.84 +0.0 39,640 + GFF680 0.85 -0.3 39,641 - GFF680 0.85 -0.6 39,712 - -0.4 39,745 - GFF681 0.16 -0.6 40,403 + +0.7 40,403 + +0.1 40,510 + +0.2 40,510 + +1.6 40,510 + +0.6 40,511 - -0.2 40,526 - GFF683 0.10 +0.1 40,550 + GFF683 0.14 +0.1 40,585 - GFF683 0.19 +0.1 40,687 - GFF683 0.33 -1.9
Or see this region's nucleotide sequence