Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF670 and GFF671 are separated by 86 nucleotides GFF671 and GFF672 overlap by 4 nucleotides
GFF670 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 25,858 to 27,147
GFF670
GFF671 - Biotin synthase (EC 2.8.1.6), at 27,234 to 28,274
GFF671
GFF672 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 28,271 to 29,428
GFF672
Position (kb) 27
28
29 Strain fitness (log2 ratio) -1
0
1 at 26.235 kb on + strand, within GFF670 at 26.236 kb on - strand, within GFF670 at 26.408 kb on - strand, within GFF670 at 26.451 kb on + strand, within GFF670 at 26.452 kb on - strand, within GFF670 at 26.452 kb on - strand, within GFF670 at 26.458 kb on - strand, within GFF670 at 26.480 kb on + strand, within GFF670 at 26.480 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.482 kb on + strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.483 kb on - strand, within GFF670 at 26.869 kb on - strand, within GFF670 at 26.971 kb on - strand, within GFF670 at 26.971 kb on - strand, within GFF670 at 26.973 kb on - strand, within GFF670 at 27.069 kb on - strand at 27.099 kb on - strand at 27.217 kb on + strand at 27.353 kb on - strand, within GFF671 at 27.362 kb on + strand, within GFF671 at 27.363 kb on - strand, within GFF671 at 27.404 kb on + strand, within GFF671 at 27.405 kb on - strand, within GFF671 at 27.467 kb on - strand, within GFF671 at 27.476 kb on - strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.723 kb on - strand, within GFF671 at 27.738 kb on - strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.742 kb on - strand, within GFF671 at 27.788 kb on + strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.151 kb on + strand, within GFF671 at 28.254 kb on + strand at 28.255 kb on - strand at 28.324 kb on + strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.391 kb on + strand, within GFF672 at 28.391 kb on + strand, within GFF672 at 28.392 kb on - strand, within GFF672 at 28.397 kb on - strand, within GFF672 at 28.425 kb on + strand, within GFF672 at 28.585 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 26,235 + GFF670 0.29 +0.1 26,236 - GFF670 0.29 -0.2 26,408 - GFF670 0.43 +0.4 26,451 + GFF670 0.46 +0.0 26,452 - GFF670 0.46 +1.7 26,452 - GFF670 0.46 +0.2 26,458 - GFF670 0.47 +0.1 26,480 + GFF670 0.48 -0.4 26,480 + GFF670 0.48 -0.9 26,482 + GFF670 0.48 +0.3 26,482 + GFF670 0.48 +0.3 26,482 + GFF670 0.48 +0.0 26,483 - GFF670 0.48 +1.2 26,483 - GFF670 0.48 -0.2 26,483 - GFF670 0.48 +0.5 26,483 - GFF670 0.48 -0.8 26,869 - GFF670 0.78 -0.6 26,971 - GFF670 0.86 +0.0 26,971 - GFF670 0.86 +0.1 26,973 - GFF670 0.86 +0.1 27,069 - -0.9 27,099 - -0.1 27,217 + -0.5 27,353 - GFF671 0.11 -0.3 27,362 + GFF671 0.12 -0.1 27,363 - GFF671 0.12 -0.2 27,404 + GFF671 0.16 -0.4 27,405 - GFF671 0.16 +0.1 27,467 - GFF671 0.22 -0.3 27,476 - GFF671 0.23 -0.4 27,681 + GFF671 0.43 +0.1 27,681 + GFF671 0.43 -0.4 27,723 - GFF671 0.47 +0.3 27,738 - GFF671 0.48 -0.3 27,741 + GFF671 0.49 +0.6 27,741 + GFF671 0.49 +0.1 27,742 - GFF671 0.49 +0.0 27,788 + GFF671 0.53 +1.5 27,789 - GFF671 0.53 +0.8 27,789 - GFF671 0.53 -0.8 28,005 + GFF671 0.74 +0.5 28,005 + GFF671 0.74 -0.7 28,151 + GFF671 0.88 +0.6 28,254 + -0.2 28,255 - -0.4 28,324 + -0.5 28,341 - +0.1 28,341 - +0.9 28,391 + GFF672 0.10 +0.1 28,391 + GFF672 0.10 -0.3 28,392 - GFF672 0.10 -0.4 28,397 - GFF672 0.11 -1.1 28,425 + GFF672 0.13 -0.2 28,585 + GFF672 0.27 -0.5 28,802 + GFF672 0.46 +0.3 28,802 + GFF672 0.46 -0.2 28,802 + GFF672 0.46 +0.1 28,802 + GFF672 0.46 +0.0 28,803 - GFF672 0.46 +0.2 28,803 - GFF672 0.46 -0.2 28,803 - GFF672 0.46 +0.2
Or see this region's nucleotide sequence