Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF645

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF644 and GFF645 are separated by 118 nucleotidesGFF645 and GFF646 are separated by 475 nucleotides GFF644 - Oxaloacetate decarboxylase beta chain (EC 4.1.1.3), at 21 to 284 GFF644 GFF645 - Membrane protein associated with oxaloacetate decarboxylase, at 403 to 1,101 GFF645 GFF646 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,577 to 2,560 GFF646 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1at 0.268 kb on - strandat 0.283 kb on - strandat 0.390 kb on + strandat 0.390 kb on + strandat 0.467 kb on - strandat 0.468 kb on + strandat 0.725 kb on + strand, within GFF645at 1.123 kb on + strandat 1.123 kb on + strandat 1.199 kb on - strandat 1.366 kb on - strandat 1.366 kb on - strandat 1.447 kb on + strandat 1.506 kb on + strandat 1.581 kb on + strandat 1.701 kb on + strand, within GFF646at 1.888 kb on + strand, within GFF646at 1.969 kb on + strand, within GFF646at 1.969 kb on + strand, within GFF646at 2.057 kb on - strand, within GFF646

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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268 - -0.2
283 - -0.5
390 + -0.1
390 + +0.4
467 - -0.5
468 + -0.5
725 + GFF645 0.46 -0.8
1,123 + +0.0
1,123 + -1.4
1,199 - -0.8
1,366 - -0.9
1,366 - +0.2
1,447 + +0.2
1,506 + -0.5
1,581 + -0.1
1,701 + GFF646 0.13 -0.9
1,888 + GFF646 0.32 +0.4
1,969 + GFF646 0.40 -0.4
1,969 + GFF646 0.40 +0.3
2,057 - GFF646 0.49 -0.2

Or see this region's nucleotide sequence