Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4916

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4914 and GFF4915 overlap by 4 nucleotidesGFF4915 and GFF4916 overlap by 4 nucleotidesGFF4916 and GFF4917 are separated by 400 nucleotides GFF4914 - Putative nucleotide di-P-sugar epimerase or dehydratase, at 17,549 to 18,562 GFF4914 GFF4915 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 18,559 to 19,389 GFF4915 GFF4916 - FIG00639119: hypothetical protein, at 19,386 to 19,709 GFF4916 GFF4917 - hypothetical protein, at 20,110 to 20,247 GFF4917 Position (kb) 19 20Strain fitness (log2 ratio) -2 -1 0 1at 18.445 kb on + strand, within GFF4914at 18.572 kb on + strandat 18.573 kb on - strandat 18.710 kb on + strand, within GFF4915at 18.711 kb on - strand, within GFF4915at 18.921 kb on + strand, within GFF4915at 18.921 kb on + strand, within GFF4915at 18.934 kb on - strand, within GFF4915at 18.937 kb on - strand, within GFF4915at 19.051 kb on - strand, within GFF4915at 19.149 kb on - strand, within GFF4915at 19.149 kb on - strand, within GFF4915at 19.175 kb on + strand, within GFF4915at 19.434 kb on - strand, within GFF4916at 19.434 kb on - strand, within GFF4916at 19.460 kb on - strand, within GFF4916at 19.731 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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18,445 + GFF4914 0.88 -0.5
18,572 + -0.5
18,573 - +0.3
18,710 + GFF4915 0.18 +0.6
18,711 - GFF4915 0.18 -1.8
18,921 + GFF4915 0.44 -0.7
18,921 + GFF4915 0.44 -0.8
18,934 - GFF4915 0.45 -0.2
18,937 - GFF4915 0.45 -0.1
19,051 - GFF4915 0.59 -0.1
19,149 - GFF4915 0.71 -0.4
19,149 - GFF4915 0.71 -0.2
19,175 + GFF4915 0.74 +0.0
19,434 - GFF4916 0.15 -1.5
19,434 - GFF4916 0.15 +0.3
19,460 - GFF4916 0.23 -2.1
19,731 - +0.4

Or see this region's nucleotide sequence