Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4863 and GFF4864 overlap by 8 nucleotides GFF4864 and GFF4865 are separated by 18 nucleotides GFF4865 and GFF4866 are separated by 346 nucleotides GFF4866 and GFF4867 are separated by 192 nucleotides
GFF4863 - 'LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM' transl_table=11, at 162,730 to 163,830
GFF4863
GFF4864 - COG2363, at 163,823 to 164,218
GFF4864
GFF4865 - Glycine cleavage system transcriptional activator GcvA, at 164,237 to 165,154
GFF4865
GFF4866 - probable lipoprotein, at 165,501 to 165,728
GFF4866
GFF4867 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 165,921 to 167,126
GFF4867
Position (kb) 164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 163.314 kb on + strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.492 kb on - strand, within GFF4863 at 163.691 kb on + strand, within GFF4863 at 163.701 kb on - strand, within GFF4863 at 163.817 kb on - strand at 163.824 kb on + strand at 163.824 kb on + strand at 163.844 kb on - strand at 163.852 kb on + strand at 164.173 kb on + strand, within GFF4864 at 164.174 kb on - strand, within GFF4864 at 164.203 kb on + strand at 164.225 kb on - strand at 164.229 kb on + strand at 164.352 kb on + strand, within GFF4865 at 164.429 kb on - strand, within GFF4865 at 164.435 kb on - strand, within GFF4865 at 164.495 kb on - strand, within GFF4865 at 164.495 kb on - strand, within GFF4865 at 164.499 kb on - strand, within GFF4865 at 164.518 kb on + strand, within GFF4865 at 164.521 kb on + strand, within GFF4865 at 164.545 kb on + strand, within GFF4865 at 164.547 kb on + strand, within GFF4865 at 164.548 kb on - strand, within GFF4865 at 164.582 kb on + strand, within GFF4865 at 164.661 kb on - strand, within GFF4865 at 164.807 kb on - strand, within GFF4865 at 164.899 kb on + strand, within GFF4865 at 164.936 kb on - strand, within GFF4865 at 165.656 kb on - strand, within GFF4866 at 165.696 kb on - strand, within GFF4866 at 165.755 kb on + strand at 165.820 kb on - strand at 165.823 kb on + strand at 165.838 kb on + strand at 165.838 kb on + strand at 165.857 kb on + strand at 165.871 kb on + strand at 166.113 kb on + strand, within GFF4867 at 166.147 kb on + strand, within GFF4867
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 163,314 + GFF4863 0.53 +0.2 163,356 - GFF4863 0.57 -1.1 163,356 - GFF4863 0.57 +0.0 163,492 - GFF4863 0.69 -0.9 163,691 + GFF4863 0.87 -0.7 163,701 - GFF4863 0.88 +1.0 163,817 - +0.2 163,824 + -0.2 163,824 + +0.1 163,844 - -0.1 163,852 + -0.2 164,173 + GFF4864 0.88 +0.3 164,174 - GFF4864 0.89 +0.4 164,203 + -1.0 164,225 - +1.7 164,229 + -0.2 164,352 + GFF4865 0.13 -1.0 164,429 - GFF4865 0.21 -0.9 164,435 - GFF4865 0.22 -3.6 164,495 - GFF4865 0.28 -0.1 164,495 - GFF4865 0.28 +1.2 164,499 - GFF4865 0.29 -0.8 164,518 + GFF4865 0.31 -0.2 164,521 + GFF4865 0.31 -0.8 164,545 + GFF4865 0.34 -0.1 164,547 + GFF4865 0.34 -2.1 164,548 - GFF4865 0.34 -0.7 164,582 + GFF4865 0.38 -1.6 164,661 - GFF4865 0.46 -0.1 164,807 - GFF4865 0.62 +0.9 164,899 + GFF4865 0.72 -1.1 164,936 - GFF4865 0.76 -0.8 165,656 - GFF4866 0.68 +0.8 165,696 - GFF4866 0.86 +1.3 165,755 + +0.4 165,820 - +0.3 165,823 + +0.4 165,838 + +0.8 165,838 + -0.5 165,857 + -0.3 165,871 + +0.4 166,113 + GFF4867 0.16 +0.4 166,147 + GFF4867 0.19 +0.3
Or see this region's nucleotide sequence