Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4775 and GFF4776 are separated by 108 nucleotides GFF4776 and GFF4777 are separated by 41 nucleotides
GFF4775 - Possible membrane transport protein, at 83,062 to 84,270
GFF4775
GFF4776 - LysR-family transcriptional regulator STM2912, at 84,379 to 85,290
GFF4776
GFF4777 - Gluconate permease, at 85,332 to 86,828
GFF4777
Position (kb) 84
85
86 Strain fitness (log2 ratio) -2
-1
0
1
2 at 83.416 kb on + strand, within GFF4775 at 83.426 kb on - strand, within GFF4775 at 83.589 kb on - strand, within GFF4775 at 83.623 kb on - strand, within GFF4775 at 83.941 kb on + strand, within GFF4775 at 83.967 kb on + strand, within GFF4775 at 83.990 kb on - strand, within GFF4775 at 84.121 kb on - strand, within GFF4775 at 84.123 kb on - strand, within GFF4775 at 84.159 kb on + strand at 84.159 kb on + strand at 84.159 kb on + strand at 84.159 kb on + strand at 84.160 kb on - strand at 84.239 kb on + strand at 84.263 kb on + strand at 84.263 kb on + strand at 84.272 kb on - strand at 84.321 kb on + strand at 84.341 kb on - strand at 84.342 kb on + strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.397 kb on - strand at 84.420 kb on - strand at 84.521 kb on + strand, within GFF4776 at 84.527 kb on + strand, within GFF4776 at 84.664 kb on - strand, within GFF4776 at 84.959 kb on + strand, within GFF4776 at 84.990 kb on + strand, within GFF4776 at 85.020 kb on - strand, within GFF4776 at 85.214 kb on + strand at 85.214 kb on + strand at 85.215 kb on - strand at 85.326 kb on - strand at 85.333 kb on + strand at 85.334 kb on - strand at 85.334 kb on - strand at 85.391 kb on + strand at 85.640 kb on + strand, within GFF4777 at 85.640 kb on + strand, within GFF4777 at 85.641 kb on - strand, within GFF4777 at 85.910 kb on + strand, within GFF4777 at 85.911 kb on - strand, within GFF4777 at 85.937 kb on + strand, within GFF4777 at 85.940 kb on - strand, within GFF4777 at 86.041 kb on - strand, within GFF4777 at 86.150 kb on + strand, within GFF4777 at 86.155 kb on + strand, within GFF4777 at 86.156 kb on - strand, within GFF4777 at 86.240 kb on - strand, within GFF4777
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 83,416 + GFF4775 0.29 -0.3 83,426 - GFF4775 0.30 -0.1 83,589 - GFF4775 0.44 -0.1 83,623 - GFF4775 0.46 -0.1 83,941 + GFF4775 0.73 -0.2 83,967 + GFF4775 0.75 -0.0 83,990 - GFF4775 0.77 +0.2 84,121 - GFF4775 0.88 +0.6 84,123 - GFF4775 0.88 -1.8 84,159 + -1.0 84,159 + +0.3 84,159 + +0.1 84,159 + +0.6 84,160 - +0.2 84,239 + -0.8 84,263 + +0.2 84,263 + +0.7 84,272 - -0.3 84,321 + +0.0 84,341 - -0.2 84,342 + -0.4 84,343 - +0.1 84,343 - -0.8 84,343 - -0.1 84,397 - +0.7 84,420 - -0.1 84,521 + GFF4776 0.16 -0.0 84,527 + GFF4776 0.16 -0.8 84,664 - GFF4776 0.31 -0.7 84,959 + GFF4776 0.64 -0.0 84,990 + GFF4776 0.67 -1.2 85,020 - GFF4776 0.70 -0.5 85,214 + +0.7 85,214 + +0.2 85,215 - -0.9 85,326 - -0.4 85,333 + +0.5 85,334 - -0.2 85,334 - -0.7 85,391 + -0.2 85,640 + GFF4777 0.21 +0.1 85,640 + GFF4777 0.21 +1.8 85,641 - GFF4777 0.21 -0.1 85,910 + GFF4777 0.39 +0.5 85,911 - GFF4777 0.39 +0.2 85,937 + GFF4777 0.40 +0.2 85,940 - GFF4777 0.41 -0.3 86,041 - GFF4777 0.47 -0.9 86,150 + GFF4777 0.55 -0.0 86,155 + GFF4777 0.55 -0.4 86,156 - GFF4777 0.55 -1.6 86,240 - GFF4777 0.61 -0.3
Or see this region's nucleotide sequence