Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4729 and GFF4730 overlap by 10 nucleotides GFF4730 and GFF4731 are separated by 98 nucleotides GFF4731 and GFF4732 are separated by 202 nucleotides
GFF4729 - Manganese ABC transporter, inner membrane permease protein SitC, at 37,883 to 38,743
GFF4729
GFF4730 - Manganese ABC transporter, inner membrane permease protein SitD, at 38,734 to 39,582
GFF4730
GFF4731 - Type III secretion injected virulence protein (YopP,YopJ, induces apoptosis, prevents cytokine induction, inhibits NFkb activation), at 39,681 to 40,547
GFF4731
GFF4732 - SPI1-associated transcriptional regulator SprB, at 40,750 to 41,505
GFF4732
Position (kb) 39
40
41 Strain fitness (log2 ratio) -1
0
1 at 38.775 kb on + strand at 38.825 kb on + strand, within GFF4730 at 38.825 kb on + strand, within GFF4730 at 38.847 kb on - strand, within GFF4730 at 38.911 kb on + strand, within GFF4730 at 38.911 kb on + strand, within GFF4730 at 38.912 kb on - strand, within GFF4730 at 38.913 kb on + strand, within GFF4730 at 38.914 kb on - strand, within GFF4730 at 38.914 kb on - strand, within GFF4730 at 39.065 kb on - strand, within GFF4730 at 39.114 kb on + strand, within GFF4730 at 39.182 kb on + strand, within GFF4730 at 39.268 kb on + strand, within GFF4730 at 39.279 kb on - strand, within GFF4730 at 39.282 kb on + strand, within GFF4730 at 39.298 kb on - strand, within GFF4730 at 39.612 kb on - strand at 39.612 kb on - strand at 39.811 kb on - strand, within GFF4731 at 39.845 kb on - strand, within GFF4731 at 39.873 kb on - strand, within GFF4731 at 40.431 kb on - strand, within GFF4731 at 40.806 kb on + strand at 41.098 kb on - strand, within GFF4732
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 38,775 + +0.1 38,825 + GFF4730 0.11 +0.6 38,825 + GFF4730 0.11 -0.3 38,847 - GFF4730 0.13 -0.3 38,911 + GFF4730 0.21 -0.2 38,911 + GFF4730 0.21 -0.3 38,912 - GFF4730 0.21 -0.4 38,913 + GFF4730 0.21 -0.4 38,914 - GFF4730 0.21 -0.3 38,914 - GFF4730 0.21 -0.7 39,065 - GFF4730 0.39 +0.5 39,114 + GFF4730 0.45 -0.3 39,182 + GFF4730 0.53 -0.2 39,268 + GFF4730 0.63 -0.1 39,279 - GFF4730 0.64 +0.8 39,282 + GFF4730 0.65 -1.7 39,298 - GFF4730 0.66 +0.3 39,612 - -0.5 39,612 - +0.5 39,811 - GFF4731 0.15 -1.3 39,845 - GFF4731 0.19 -0.4 39,873 - GFF4731 0.22 -1.2 40,431 - GFF4731 0.87 +0.1 40,806 + -0.5 41,098 - GFF4732 0.46 -0.4
Or see this region's nucleotide sequence