Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4654 and GFF4655 are separated by 34 nucleotides GFF4655 and GFF4656 are separated by 9 nucleotides
GFF4654 - tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins, at 446,640 to 447,935
GFF4654
GFF4655 - 18K peptidoglycan-associated outer membrane lipoprotein; Peptidoglycan-associated lipoprotein precursor; Outer membrane protein P6; OmpA/MotB precursor, at 447,970 to 448,494
GFF4655
GFF4656 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 448,504 to 449,292
GFF4656
Position (kb) 447
448
449 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 447.026 kb on - strand, within GFF4654 at 447.147 kb on + strand, within GFF4654 at 447.148 kb on - strand, within GFF4654 at 447.211 kb on - strand, within GFF4654 at 447.289 kb on - strand, within GFF4654 at 447.453 kb on + strand, within GFF4654 at 447.454 kb on - strand, within GFF4654 at 447.554 kb on + strand, within GFF4654 at 447.554 kb on + strand, within GFF4654 at 447.623 kb on + strand, within GFF4654 at 447.623 kb on + strand, within GFF4654 at 447.671 kb on + strand, within GFF4654 at 447.794 kb on + strand, within GFF4654 at 447.814 kb on - strand at 447.814 kb on - strand at 448.102 kb on - strand, within GFF4655 at 448.205 kb on - strand, within GFF4655 at 448.248 kb on + strand, within GFF4655 at 448.249 kb on - strand, within GFF4655 at 448.260 kb on + strand, within GFF4655 at 448.489 kb on + strand at 448.785 kb on + strand, within GFF4656 at 448.809 kb on + strand, within GFF4656 at 448.936 kb on + strand, within GFF4656 at 449.071 kb on + strand, within GFF4656 at 449.072 kb on - strand, within GFF4656 at 449.104 kb on + strand, within GFF4656 at 449.161 kb on + strand, within GFF4656 at 449.162 kb on - strand, within GFF4656 at 449.218 kb on + strand at 449.240 kb on - strand at 449.297 kb on + strand at 449.406 kb on + strand at 449.430 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 447,026 - GFF4654 0.30 -1.9 447,147 + GFF4654 0.39 +0.9 447,148 - GFF4654 0.39 +0.7 447,211 - GFF4654 0.44 +0.7 447,289 - GFF4654 0.50 -0.3 447,453 + GFF4654 0.63 -0.2 447,454 - GFF4654 0.63 +0.1 447,554 + GFF4654 0.71 -1.2 447,554 + GFF4654 0.71 -1.0 447,623 + GFF4654 0.76 -0.3 447,623 + GFF4654 0.76 +0.4 447,671 + GFF4654 0.80 +0.1 447,794 + GFF4654 0.89 +0.8 447,814 - -1.1 447,814 - +1.7 448,102 - GFF4655 0.25 -1.1 448,205 - GFF4655 0.45 -3.2 448,248 + GFF4655 0.53 -0.8 448,249 - GFF4655 0.53 -0.6 448,260 + GFF4655 0.55 +0.5 448,489 + +0.5 448,785 + GFF4656 0.36 -0.8 448,809 + GFF4656 0.39 -1.4 448,936 + GFF4656 0.55 +0.7 449,071 + GFF4656 0.72 -0.1 449,072 - GFF4656 0.72 -0.2 449,104 + GFF4656 0.76 -0.1 449,161 + GFF4656 0.83 +0.0 449,162 - GFF4656 0.83 -0.9 449,218 + +0.0 449,240 - -0.2 449,297 + +0.4 449,406 + -0.0 449,430 + +1.9
Or see this region's nucleotide sequence