Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4651 and GFF4652 are separated by 3 nucleotides GFF4652 and GFF4653 are separated by 64 nucleotides GFF4653 and GFF4654 are separated by 120 nucleotides
GFF4651 - MotA/TolQ/ExbB proton channel family protein, at 443,972 to 444,664
GFF4651
GFF4652 - Tol biopolymer transport system, TolR protein, at 444,668 to 445,096
GFF4652
GFF4653 - TolA protein, at 445,161 to 446,519
GFF4653
GFF4654 - tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins, at 446,640 to 447,935
GFF4654
Position (kb) 445
446
447 Strain fitness (log2 ratio) -2
-1
0
1 at 444.376 kb on + strand, within GFF4651 at 444.376 kb on + strand, within GFF4651 at 444.376 kb on + strand, within GFF4651 at 444.409 kb on - strand, within GFF4651 at 444.662 kb on + strand at 444.703 kb on + strand at 444.719 kb on - strand, within GFF4652 at 444.739 kb on - strand, within GFF4652 at 444.764 kb on - strand, within GFF4652 at 444.841 kb on + strand, within GFF4652 at 444.862 kb on + strand, within GFF4652 at 444.888 kb on - strand, within GFF4652 at 444.951 kb on + strand, within GFF4652 at 445.234 kb on + strand at 445.235 kb on - strand at 445.271 kb on + strand at 445.350 kb on + strand, within GFF4653 at 446.261 kb on + strand, within GFF4653 at 446.351 kb on + strand, within GFF4653 at 446.354 kb on + strand, within GFF4653 at 446.378 kb on - strand, within GFF4653 at 446.518 kb on - strand at 446.540 kb on + strand at 446.557 kb on + strand at 446.639 kb on + strand at 447.026 kb on - strand, within GFF4654 at 447.147 kb on + strand, within GFF4654 at 447.148 kb on - strand, within GFF4654 at 447.211 kb on - strand, within GFF4654 at 447.289 kb on - strand, within GFF4654 at 447.453 kb on + strand, within GFF4654 at 447.454 kb on - strand, within GFF4654
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 444,376 + GFF4651 0.58 -1.4 444,376 + GFF4651 0.58 -1.9 444,376 + GFF4651 0.58 -0.9 444,409 - GFF4651 0.63 -2.2 444,662 + +0.1 444,703 + -0.8 444,719 - GFF4652 0.12 -0.6 444,739 - GFF4652 0.17 +1.1 444,764 - GFF4652 0.22 -1.0 444,841 + GFF4652 0.40 -0.9 444,862 + GFF4652 0.45 -0.9 444,888 - GFF4652 0.51 -1.0 444,951 + GFF4652 0.66 -1.6 445,234 + -2.2 445,235 - +0.1 445,271 + -0.3 445,350 + GFF4653 0.14 +0.1 446,261 + GFF4653 0.81 -1.4 446,351 + GFF4653 0.88 -1.2 446,354 + GFF4653 0.88 +0.5 446,378 - GFF4653 0.90 -1.4 446,518 - +0.6 446,540 + +0.4 446,557 + -0.1 446,639 + -0.7 447,026 - GFF4654 0.30 -1.9 447,147 + GFF4654 0.39 +0.9 447,148 - GFF4654 0.39 +0.7 447,211 - GFF4654 0.44 +0.7 447,289 - GFF4654 0.50 -0.3 447,453 + GFF4654 0.63 -0.2 447,454 - GFF4654 0.63 +0.1
Or see this region's nucleotide sequence