Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4652

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4650 and GFF4651 overlap by 4 nucleotidesGFF4651 and GFF4652 are separated by 3 nucleotidesGFF4652 and GFF4653 are separated by 64 nucleotides GFF4650 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 443,634 to 443,975 GFF4650 GFF4651 - MotA/TolQ/ExbB proton channel family protein, at 443,972 to 444,664 GFF4651 GFF4652 - Tol biopolymer transport system, TolR protein, at 444,668 to 445,096 GFF4652 GFF4653 - TolA protein, at 445,161 to 446,519 GFF4653 Position (kb) 444 445 446Strain fitness (log2 ratio) -2 -1 0 1at 443.769 kb on + strand, within GFF4650at 443.793 kb on - strand, within GFF4650at 443.881 kb on + strand, within GFF4650at 443.945 kb on + strandat 444.376 kb on + strand, within GFF4651at 444.376 kb on + strand, within GFF4651at 444.376 kb on + strand, within GFF4651at 444.409 kb on - strand, within GFF4651at 444.662 kb on + strandat 444.703 kb on + strandat 444.719 kb on - strand, within GFF4652at 444.739 kb on - strand, within GFF4652at 444.764 kb on - strand, within GFF4652at 444.841 kb on + strand, within GFF4652at 444.862 kb on + strand, within GFF4652at 444.888 kb on - strand, within GFF4652at 444.951 kb on + strand, within GFF4652at 445.234 kb on + strandat 445.235 kb on - strandat 445.271 kb on + strandat 445.350 kb on + strand, within GFF4653

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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443,769 + GFF4650 0.39 -0.0
443,793 - GFF4650 0.46 +0.5
443,881 + GFF4650 0.72 -0.2
443,945 + -1.1
444,376 + GFF4651 0.58 -1.4
444,376 + GFF4651 0.58 -1.9
444,376 + GFF4651 0.58 -0.9
444,409 - GFF4651 0.63 -2.2
444,662 + +0.1
444,703 + -0.8
444,719 - GFF4652 0.12 -0.6
444,739 - GFF4652 0.17 +1.1
444,764 - GFF4652 0.22 -1.0
444,841 + GFF4652 0.40 -0.9
444,862 + GFF4652 0.45 -0.9
444,888 - GFF4652 0.51 -1.0
444,951 + GFF4652 0.66 -1.6
445,234 + -2.2
445,235 - +0.1
445,271 + -0.3
445,350 + GFF4653 0.14 +0.1

Or see this region's nucleotide sequence