Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4575 and GFF4576 are separated by 157 nucleotides GFF4576 and GFF4577 overlap by 4 nucleotides GFF4577 and GFF4578 are separated by 58 nucleotides GFF4578 and GFF4579 are separated by 26 nucleotides
GFF4575 - Magnesium and cobalt efflux protein CorC, at 361,944 to 362,822
GFF4575
GFF4576 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 362,980 to 363,453
GFF4576
GFF4577 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 363,450 to 364,496
GFF4577
GFF4578 - hypothetical protein, at 364,555 to 364,674
GFF4578
GFF4579 - tRNA-i(6)A37 methylthiotransferase, at 364,701 to 366,125
GFF4579
Position (kb)
363
364
365 Strain fitness (log2 ratio)
-2
-1
0
1 at 362.607 kb on - strand, within GFF4575 at 362.607 kb on - strand, within GFF4575 at 362.853 kb on - strand at 362.871 kb on - strand at 362.871 kb on - strand at 362.873 kb on - strand at 362.924 kb on - strand at 363.006 kb on - strand at 363.796 kb on - strand, within GFF4577 at 363.796 kb on - strand, within GFF4577 at 363.991 kb on - strand, within GFF4577 at 364.298 kb on - strand, within GFF4577 at 364.489 kb on - strand at 364.845 kb on - strand, within GFF4579 at 364.865 kb on + strand, within GFF4579 at 364.865 kb on + strand, within GFF4579 at 364.988 kb on + strand, within GFF4579 at 365.021 kb on + strand, within GFF4579 at 365.117 kb on + strand, within GFF4579 at 365.117 kb on + strand, within GFF4579 at 365.118 kb on - strand, within GFF4579 at 365.153 kb on + strand, within GFF4579 at 365.270 kb on - strand, within GFF4579 at 365.372 kb on - strand, within GFF4579 at 365.374 kb on - strand, within GFF4579 at 365.381 kb on + strand, within GFF4579 at 365.384 kb on + strand, within GFF4579 at 365.478 kb on - strand, within GFF4579
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 362,607 - GFF4575 0.75 -0.6 362,607 - GFF4575 0.75 -0.6 362,853 - -0.0 362,871 - +0.2 362,871 - -0.1 362,873 - -0.1 362,924 - +0.1 363,006 - +0.6 363,796 - GFF4577 0.33 +0.6 363,796 - GFF4577 0.33 +0.6 363,991 - GFF4577 0.52 +0.6 364,298 - GFF4577 0.81 -1.1 364,489 - -0.9 364,845 - GFF4579 0.10 -0.9 364,865 + GFF4579 0.12 -1.1 364,865 + GFF4579 0.12 +1.1 364,988 + GFF4579 0.20 +0.1 365,021 + GFF4579 0.22 -0.3 365,117 + GFF4579 0.29 -2.0 365,117 + GFF4579 0.29 -0.6 365,118 - GFF4579 0.29 -0.9 365,153 + GFF4579 0.32 +0.0 365,270 - GFF4579 0.40 -0.1 365,372 - GFF4579 0.47 +0.3 365,374 - GFF4579 0.47 -0.4 365,381 + GFF4579 0.48 -0.3 365,384 + GFF4579 0.48 -0.6 365,478 - GFF4579 0.55 -0.9
Or see this region's nucleotide sequence