Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4535 and GFF4536 are separated by 187 nucleotides GFF4536 and GFF4537 are separated by 57 nucleotides GFF4537 and GFF4538 are separated by 150 nucleotides
GFF4535 - Lipid A acylation protein PagP, palmitoyltransferase, at 322,381 to 322,941
GFF4535
GFF4536 - Cold shock protein CspE, at 323,129 to 323,338
GFF4536
GFF4537 - CrcB protein, at 323,396 to 323,719
GFF4537
GFF4538 - Aliphatic amidase AmiE (EC 3.5.1.4), at 323,870 to 324,658
GFF4538
Position (kb) 323
324 Strain fitness (log2 ratio) -2
-1
0
1 at 322.531 kb on + strand, within GFF4535 at 322.531 kb on + strand, within GFF4535 at 322.664 kb on - strand, within GFF4535 at 322.838 kb on + strand, within GFF4535 at 322.852 kb on + strand, within GFF4535 at 322.897 kb on - strand at 323.135 kb on - strand at 323.325 kb on + strand at 323.343 kb on - strand at 323.639 kb on + strand, within GFF4537 at 323.642 kb on - strand, within GFF4537 at 323.775 kb on - strand at 323.775 kb on - strand at 323.796 kb on + strand at 323.805 kb on + strand at 323.870 kb on - strand at 323.908 kb on - strand at 324.092 kb on - strand, within GFF4538 at 324.148 kb on + strand, within GFF4538 at 324.148 kb on + strand, within GFF4538 at 324.209 kb on + strand, within GFF4538 at 324.210 kb on - strand, within GFF4538 at 324.242 kb on - strand, within GFF4538 at 324.302 kb on - strand, within GFF4538 at 324.312 kb on - strand, within GFF4538 at 324.339 kb on + strand, within GFF4538 at 324.458 kb on + strand, within GFF4538 at 324.459 kb on - strand, within GFF4538 at 324.461 kb on - strand, within GFF4538 at 324.494 kb on - strand, within GFF4538 at 324.508 kb on + strand, within GFF4538 at 324.652 kb on - strand at 324.654 kb on + strand at 324.655 kb on - strand at 324.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 322,531 + GFF4535 0.27 -0.5 322,531 + GFF4535 0.27 -0.2 322,664 - GFF4535 0.50 +0.8 322,838 + GFF4535 0.81 -0.4 322,852 + GFF4535 0.84 -0.4 322,897 - +0.2 323,135 - +0.4 323,325 + -2.2 323,343 - +0.1 323,639 + GFF4537 0.75 -0.9 323,642 - GFF4537 0.76 -0.9 323,775 - -0.8 323,775 - -0.9 323,796 + +0.6 323,805 + -0.6 323,870 - -0.9 323,908 - +0.4 324,092 - GFF4538 0.28 +0.1 324,148 + GFF4538 0.35 +0.1 324,148 + GFF4538 0.35 -0.4 324,209 + GFF4538 0.43 -0.9 324,210 - GFF4538 0.43 +0.9 324,242 - GFF4538 0.47 -1.4 324,302 - GFF4538 0.55 +1.1 324,312 - GFF4538 0.56 -0.3 324,339 + GFF4538 0.59 +0.1 324,458 + GFF4538 0.75 -0.4 324,459 - GFF4538 0.75 -0.2 324,461 - GFF4538 0.75 -1.6 324,494 - GFF4538 0.79 -0.7 324,508 + GFF4538 0.81 +1.5 324,652 - +0.0 324,654 + -0.1 324,655 - -0.3 324,701 + -1.0
Or see this region's nucleotide sequence