Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4471 and GFF4472 are separated by 41 nucleotides GFF4472 and GFF4473 are separated by 26 nucleotides GFF4473 and GFF4474 are separated by 98 nucleotides
GFF4471 - Phenylalanine-specific permease, at 254,413 to 255,807
GFF4471
GFF4472 - Uncharacterized protein ybdG, at 255,849 to 257,096
GFF4472
GFF4473 - FIG01046818: hypothetical protein, at 257,123 to 257,308
GFF4473
GFF4474 - Outer membrane esterase, at 257,407 to 259,377
GFF4474
Position (kb) 255
256
257
258 Strain fitness (log2 ratio) -1
0
1 at 255.025 kb on - strand, within GFF4471 at 255.025 kb on - strand, within GFF4471 at 255.078 kb on + strand, within GFF4471 at 255.173 kb on + strand, within GFF4471 at 255.173 kb on + strand, within GFF4471 at 255.173 kb on + strand, within GFF4471 at 255.174 kb on - strand, within GFF4471 at 255.193 kb on + strand, within GFF4471 at 255.502 kb on + strand, within GFF4471 at 255.503 kb on - strand, within GFF4471 at 255.503 kb on - strand, within GFF4471 at 255.558 kb on - strand, within GFF4471 at 255.711 kb on + strand at 255.762 kb on - strand at 255.781 kb on - strand at 255.851 kb on - strand at 255.908 kb on + strand at 255.909 kb on - strand at 256.006 kb on + strand, within GFF4472 at 256.006 kb on + strand, within GFF4472 at 256.050 kb on + strand, within GFF4472 at 256.092 kb on + strand, within GFF4472 at 256.224 kb on + strand, within GFF4472 at 256.278 kb on - strand, within GFF4472 at 256.394 kb on + strand, within GFF4472 at 256.394 kb on + strand, within GFF4472 at 256.433 kb on + strand, within GFF4472 at 256.459 kb on + strand, within GFF4472 at 256.894 kb on - strand, within GFF4472 at 256.899 kb on - strand, within GFF4472 at 256.965 kb on + strand, within GFF4472 at 256.968 kb on + strand, within GFF4472 at 257.145 kb on + strand, within GFF4473 at 257.224 kb on - strand, within GFF4473 at 257.344 kb on + strand at 257.375 kb on + strand at 257.379 kb on + strand at 257.394 kb on + strand at 257.493 kb on + strand at 257.494 kb on - strand at 257.515 kb on - strand at 257.529 kb on + strand at 257.749 kb on + strand, within GFF4474 at 257.749 kb on + strand, within GFF4474 at 257.750 kb on - strand, within GFF4474 at 257.750 kb on - strand, within GFF4474 at 257.901 kb on - strand, within GFF4474
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 255,025 - GFF4471 0.44 -0.7 255,025 - GFF4471 0.44 +0.6 255,078 + GFF4471 0.48 -0.2 255,173 + GFF4471 0.54 -0.2 255,173 + GFF4471 0.54 -0.5 255,173 + GFF4471 0.54 -0.2 255,174 - GFF4471 0.55 +0.2 255,193 + GFF4471 0.56 +0.2 255,502 + GFF4471 0.78 -0.5 255,503 - GFF4471 0.78 +0.1 255,503 - GFF4471 0.78 -0.5 255,558 - GFF4471 0.82 +0.4 255,711 + -0.2 255,762 - -0.2 255,781 - +0.2 255,851 - -0.1 255,908 + -0.6 255,909 - -0.5 256,006 + GFF4472 0.13 -1.3 256,006 + GFF4472 0.13 +0.9 256,050 + GFF4472 0.16 -0.6 256,092 + GFF4472 0.19 -0.7 256,224 + GFF4472 0.30 -0.7 256,278 - GFF4472 0.34 -1.6 256,394 + GFF4472 0.44 +0.9 256,394 + GFF4472 0.44 -0.4 256,433 + GFF4472 0.47 -0.0 256,459 + GFF4472 0.49 -0.1 256,894 - GFF4472 0.84 -0.3 256,899 - GFF4472 0.84 +0.1 256,965 + GFF4472 0.89 -0.5 256,968 + GFF4472 0.90 +0.6 257,145 + GFF4473 0.12 -0.7 257,224 - GFF4473 0.54 -1.3 257,344 + -0.4 257,375 + +0.2 257,379 + +0.5 257,394 + -0.1 257,493 + +0.5 257,494 - -0.1 257,515 - -1.1 257,529 + -0.1 257,749 + GFF4474 0.17 +0.1 257,749 + GFF4474 0.17 -1.1 257,750 - GFF4474 0.17 -1.4 257,750 - GFF4474 0.17 +0.2 257,901 - GFF4474 0.25 -0.0
Or see this region's nucleotide sequence