Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4436 and GFF4437 are separated by 3 nucleotides GFF4437 and GFF4438 are separated by 40 nucleotides GFF4438 and GFF4439 overlap by 4 nucleotides GFF4439 and GFF4440 are separated by 117 nucleotides
GFF4436 - FIG074102: hypothetical protein, at 224,540 to 225,328
GFF4436
GFF4437 - Carbamate kinase (EC 2.7.2.2), at 225,332 to 226,225
GFF4437
GFF4438 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 226,266 to 227,333
GFF4438
GFF4439 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 227,330 to 227,839
GFF4439
GFF4440 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 227,957 to 228,679
GFF4440
Position (kb) 226
227
228 Strain fitness (log2 ratio) -1
0
1 at 225.346 kb on - strand at 225.369 kb on + strand at 225.415 kb on + strand at 225.415 kb on + strand at 225.415 kb on + strand at 225.471 kb on + strand, within GFF4437 at 225.475 kb on - strand, within GFF4437 at 225.475 kb on - strand, within GFF4437 at 225.589 kb on + strand, within GFF4437 at 225.589 kb on + strand, within GFF4437 at 225.713 kb on + strand, within GFF4437 at 225.749 kb on + strand, within GFF4437 at 225.779 kb on + strand, within GFF4437 at 225.931 kb on - strand, within GFF4437 at 226.013 kb on + strand, within GFF4437 at 226.014 kb on - strand, within GFF4437 at 226.267 kb on + strand at 226.271 kb on + strand at 226.361 kb on + strand at 226.362 kb on - strand at 226.421 kb on - strand, within GFF4438 at 226.435 kb on - strand, within GFF4438 at 226.630 kb on - strand, within GFF4438 at 226.678 kb on + strand, within GFF4438 at 226.679 kb on - strand, within GFF4438 at 226.883 kb on + strand, within GFF4438 at 226.884 kb on - strand, within GFF4438 at 226.894 kb on + strand, within GFF4438 at 226.977 kb on - strand, within GFF4438 at 227.527 kb on - strand, within GFF4439 at 227.723 kb on + strand, within GFF4439 at 227.724 kb on - strand, within GFF4439 at 227.724 kb on - strand, within GFF4439 at 227.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 225,346 - -0.9 225,369 + +0.0 225,415 + +0.1 225,415 + +0.6 225,415 + +0.7 225,471 + GFF4437 0.16 -0.3 225,475 - GFF4437 0.16 +1.2 225,475 - GFF4437 0.16 -0.3 225,589 + GFF4437 0.29 -0.2 225,589 + GFF4437 0.29 -0.2 225,713 + GFF4437 0.43 +0.8 225,749 + GFF4437 0.47 +0.0 225,779 + GFF4437 0.50 -0.2 225,931 - GFF4437 0.67 +0.6 226,013 + GFF4437 0.76 -0.7 226,014 - GFF4437 0.76 +0.3 226,267 + +0.8 226,271 + -0.1 226,361 + -0.1 226,362 - -0.4 226,421 - GFF4438 0.15 -1.1 226,435 - GFF4438 0.16 -0.4 226,630 - GFF4438 0.34 -0.2 226,678 + GFF4438 0.39 -0.7 226,679 - GFF4438 0.39 +1.4 226,883 + GFF4438 0.58 -0.6 226,884 - GFF4438 0.58 -0.1 226,894 + GFF4438 0.59 -1.3 226,977 - GFF4438 0.67 -0.7 227,527 - GFF4439 0.39 -0.0 227,723 + GFF4439 0.77 -0.4 227,724 - GFF4439 0.77 +0.3 227,724 - GFF4439 0.77 -0.3 227,894 - -0.3
Or see this region's nucleotide sequence