Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4416 and GFF4417 are separated by 36 nucleotides GFF4417 and GFF4418 overlap by 8 nucleotides GFF4418 and GFF4419 are separated by 38 nucleotides
GFF4416 - Methionine ABC transporter substrate-binding protein, at 201,326 to 202,156
GFF4416
GFF4417 - Methionine ABC transporter ATP-binding protein, at 202,193 to 203,209
GFF4417
GFF4418 - Methionine ABC transporter permease protein, at 203,202 to 203,861
GFF4418
GFF4419 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 203,900 to 204,994
GFF4419
Position (kb) 202
203
204 Strain fitness (log2 ratio) -1
0
1
2 at 201.312 kb on - strand at 201.423 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.483 kb on + strand, within GFF4416 at 201.499 kb on + strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.550 kb on - strand, within GFF4416 at 201.671 kb on + strand, within GFF4416 at 201.785 kb on - strand, within GFF4416 at 201.793 kb on + strand, within GFF4416 at 201.802 kb on + strand, within GFF4416 at 201.803 kb on - strand, within GFF4416 at 202.113 kb on - strand at 202.473 kb on + strand, within GFF4417 at 202.474 kb on - strand, within GFF4417 at 203.083 kb on + strand, within GFF4417 at 203.134 kb on + strand at 203.410 kb on + strand, within GFF4418 at 203.410 kb on + strand, within GFF4418 at 203.423 kb on - strand, within GFF4418 at 203.454 kb on - strand, within GFF4418 at 203.594 kb on + strand, within GFF4418 at 203.595 kb on - strand, within GFF4418 at 203.595 kb on - strand, within GFF4418 at 203.597 kb on + strand, within GFF4418 at 203.597 kb on + strand, within GFF4418 at 203.598 kb on - strand, within GFF4418 at 203.609 kb on - strand, within GFF4418 at 203.668 kb on + strand, within GFF4418 at 203.742 kb on + strand, within GFF4418 at 203.874 kb on + strand at 203.896 kb on + strand at 203.897 kb on - strand at 203.902 kb on - strand at 203.985 kb on + strand at 203.986 kb on - strand at 204.007 kb on + strand at 204.056 kb on + strand, within GFF4419 at 204.162 kb on + strand, within GFF4419
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 201,312 - -1.2 201,423 + GFF4416 0.12 -0.6 201,483 + GFF4416 0.19 +0.4 201,483 + GFF4416 0.19 -0.1 201,499 + GFF4416 0.21 -0.4 201,550 - GFF4416 0.27 +0.4 201,550 - GFF4416 0.27 -0.4 201,671 + GFF4416 0.42 -0.5 201,785 - GFF4416 0.55 +0.1 201,793 + GFF4416 0.56 +0.1 201,802 + GFF4416 0.57 +0.3 201,803 - GFF4416 0.57 -1.2 202,113 - +0.0 202,473 + GFF4417 0.28 -0.7 202,474 - GFF4417 0.28 -0.9 203,083 + GFF4417 0.88 -0.4 203,134 + +0.4 203,410 + GFF4418 0.32 -0.1 203,410 + GFF4418 0.32 -0.4 203,423 - GFF4418 0.33 -0.1 203,454 - GFF4418 0.38 +0.6 203,594 + GFF4418 0.59 +0.3 203,595 - GFF4418 0.60 -0.1 203,595 - GFF4418 0.60 +0.1 203,597 + GFF4418 0.60 +0.3 203,597 + GFF4418 0.60 -0.2 203,598 - GFF4418 0.60 -0.7 203,609 - GFF4418 0.62 +0.6 203,668 + GFF4418 0.71 -0.2 203,742 + GFF4418 0.82 -0.0 203,874 + -0.6 203,896 + -0.2 203,897 - -0.3 203,902 - +0.0 203,985 + +0.1 203,986 - -0.0 204,007 + -0.2 204,056 + GFF4419 0.14 +2.1 204,162 + GFF4419 0.24 -0.1
Or see this region's nucleotide sequence