Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4383 and GFF4384 are separated by 69 nucleotides GFF4384 and GFF4385 are separated by 13 nucleotides GFF4385 and GFF4386 are separated by 68 nucleotides GFF4386 and GFF4387 are separated by 152 nucleotides GFF4387 and GFF4388 are separated by 113 nucleotides
GFF4383 - Mobile element protein, at 168,144 to 169,079
GFF4383
GFF4384 - Putative inner membrane protein, at 169,149 to 169,316
GFF4384
GFF4385 - Primosomal replication protein N prime prime, at 169,330 to 169,857
GFF4385
GFF4386 - FIG039061: hypothetical protein related to heme utilization, at 169,926 to 170,303
GFF4386
GFF4387 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 170,456 to 171,007
GFF4387
GFF4388 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 171,121 to 173,049
GFF4388
Position (kb) 169
170
171 Strain fitness (log2 ratio) -1
0
1 at 169.183 kb on - strand, within GFF4384 at 169.205 kb on + strand, within GFF4384 at 169.205 kb on + strand, within GFF4384 at 169.224 kb on + strand, within GFF4384 at 169.270 kb on - strand, within GFF4384 at 169.270 kb on - strand, within GFF4384 at 169.316 kb on + strand at 169.317 kb on - strand at 169.352 kb on - strand at 169.403 kb on + strand, within GFF4385 at 169.404 kb on - strand, within GFF4385 at 169.404 kb on - strand, within GFF4385 at 169.837 kb on + strand at 169.838 kb on - strand at 169.892 kb on + strand at 169.935 kb on - strand at 169.937 kb on + strand at 169.937 kb on + strand at 169.969 kb on + strand, within GFF4386 at 170.078 kb on - strand, within GFF4386 at 170.168 kb on - strand, within GFF4386 at 170.184 kb on - strand, within GFF4386 at 170.437 kb on - strand at 170.437 kb on - strand at 170.449 kb on + strand at 170.449 kb on + strand at 170.449 kb on + strand at 170.449 kb on + strand at 170.450 kb on - strand at 170.450 kb on - strand at 170.727 kb on + strand, within GFF4387 at 170.754 kb on - strand, within GFF4387
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 169,183 - GFF4384 0.20 +0.7 169,205 + GFF4384 0.33 +1.7 169,205 + GFF4384 0.33 -0.5 169,224 + GFF4384 0.45 +0.1 169,270 - GFF4384 0.72 -0.6 169,270 - GFF4384 0.72 +1.3 169,316 + -0.2 169,317 - +0.3 169,352 - +0.0 169,403 + GFF4385 0.14 -0.0 169,404 - GFF4385 0.14 -0.3 169,404 - GFF4385 0.14 -0.0 169,837 + +0.7 169,838 - -0.2 169,892 + +0.3 169,935 - -1.1 169,937 + +1.0 169,937 + +0.3 169,969 + GFF4386 0.11 -0.7 170,078 - GFF4386 0.40 -0.1 170,168 - GFF4386 0.64 -1.3 170,184 - GFF4386 0.68 +0.1 170,437 - +0.7 170,437 - +0.0 170,449 + +0.3 170,449 + -0.4 170,449 + -0.2 170,449 + -0.3 170,450 - -0.6 170,450 - -0.3 170,727 + GFF4387 0.49 -0.8 170,754 - GFF4387 0.54 -1.2
Or see this region's nucleotide sequence