Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4355

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4354 and GFF4355 are separated by 185 nucleotidesGFF4355 and GFF4356 are separated by 208 nucleotidesGFF4356 and GFF4357 are separated by 290 nucleotides GFF4354 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 134,297 to 135,568 GFF4354 GFF4355 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 135,754 to 138,108 GFF4355 GFF4356 - DNA-binding protein HU-beta, at 138,317 to 138,589 GFF4356 GFF4357 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 138,880 to 140,751 GFF4357 Position (kb) 135 136 137 138 139Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 135.010 kb on - strand, within GFF4354at 135.013 kb on + strand, within GFF4354at 135.449 kb on + strandat 135.449 kb on + strandat 135.671 kb on + strandat 135.679 kb on + strandat 135.680 kb on - strandat 135.689 kb on - strandat 135.708 kb on + strandat 135.708 kb on + strandat 135.708 kb on + strandat 135.709 kb on - strandat 135.709 kb on - strandat 135.710 kb on + strandat 136.946 kb on - strand, within GFF4355at 137.440 kb on - strand, within GFF4355at 137.947 kb on - strandat 138.179 kb on + strandat 138.186 kb on - strandat 138.390 kb on + strand, within GFF4356at 138.401 kb on - strand, within GFF4356at 138.582 kb on + strandat 138.587 kb on + strandat 138.620 kb on - strandat 138.763 kb on + strandat 138.848 kb on + strandat 139.000 kb on + strandat 139.068 kb on + strand, within GFF4357at 139.074 kb on + strand, within GFF4357

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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135,010 - GFF4354 0.56 -0.5
135,013 + GFF4354 0.56 -2.0
135,449 + -0.9
135,449 + -1.2
135,671 + -0.2
135,679 + -2.1
135,680 - +0.5
135,689 - +0.7
135,708 + -1.0
135,708 + -0.9
135,708 + -2.6
135,709 - -1.1
135,709 - -1.5
135,710 + +0.1
136,946 - GFF4355 0.51 -1.1
137,440 - GFF4355 0.72 -4.0
137,947 - -0.9
138,179 + -0.0
138,186 - +0.0
138,390 + GFF4356 0.27 -0.5
138,401 - GFF4356 0.31 +0.0
138,582 + -0.4
138,587 + -1.1
138,620 - -0.2
138,763 + -0.3
138,848 + +0.1
139,000 + -0.4
139,068 + GFF4357 0.10 -1.3
139,074 + GFF4357 0.10 -0.6

Or see this region's nucleotide sequence