Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4174 and GFF4175 are separated by 73 nucleotides GFF4175 and GFF4176 are separated by 439 nucleotides
GFF4174 - Inner membrane protein YfiN, at 62,781 to 63,920
GFF4174
GFF4175 - Uncharacterized protein YfiR precursor, at 63,994 to 64,512
GFF4175
GFF4176 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 64,952 to 66,022
GFF4176
Position (kb) 63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 62.995 kb on - strand, within GFF4174 at 62.995 kb on - strand, within GFF4174 at 62.995 kb on - strand, within GFF4174 at 63.001 kb on + strand, within GFF4174 at 63.090 kb on - strand, within GFF4174 at 63.273 kb on + strand, within GFF4174 at 63.274 kb on - strand, within GFF4174 at 63.301 kb on - strand, within GFF4174 at 63.333 kb on - strand, within GFF4174 at 63.469 kb on + strand, within GFF4174 at 63.509 kb on + strand, within GFF4174 at 63.555 kb on - strand, within GFF4174 at 63.688 kb on + strand, within GFF4174 at 63.865 kb on + strand at 63.866 kb on - strand at 64.102 kb on - strand, within GFF4175 at 64.156 kb on + strand, within GFF4175 at 64.346 kb on - strand, within GFF4175 at 64.352 kb on - strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.386 kb on + strand, within GFF4175 at 64.387 kb on - strand, within GFF4175 at 64.388 kb on + strand, within GFF4175 at 64.552 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.820 kb on + strand at 64.821 kb on - strand at 64.978 kb on - strand at 64.999 kb on - strand at 65.020 kb on + strand at 65.062 kb on - strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.168 kb on + strand, within GFF4176 at 65.169 kb on - strand, within GFF4176 at 65.216 kb on + strand, within GFF4176
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 62,995 - GFF4174 0.19 +0.8 62,995 - GFF4174 0.19 +1.0 62,995 - GFF4174 0.19 +0.0 63,001 + GFF4174 0.19 -0.6 63,090 - GFF4174 0.27 +0.6 63,273 + GFF4174 0.43 -0.1 63,274 - GFF4174 0.43 +0.1 63,301 - GFF4174 0.46 -0.5 63,333 - GFF4174 0.48 -0.3 63,469 + GFF4174 0.60 +0.3 63,509 + GFF4174 0.64 +0.8 63,555 - GFF4174 0.68 +1.3 63,688 + GFF4174 0.80 +1.8 63,865 + +0.7 63,866 - +0.8 64,102 - GFF4175 0.21 -3.8 64,156 + GFF4175 0.31 +0.6 64,346 - GFF4175 0.68 -1.6 64,352 - GFF4175 0.69 -0.8 64,386 + GFF4175 0.76 +0.4 64,386 + GFF4175 0.76 -0.8 64,387 - GFF4175 0.76 -0.9 64,388 + GFF4175 0.76 -1.1 64,552 + -0.1 64,820 + -1.8 64,820 + -0.4 64,820 + +0.3 64,821 - +0.4 64,978 - -0.6 64,999 - -1.0 65,020 + +0.3 65,062 - GFF4176 0.10 -0.0 65,124 + GFF4176 0.16 +0.4 65,124 + GFF4176 0.16 -0.0 65,168 + GFF4176 0.20 +0.2 65,169 - GFF4176 0.20 -1.5 65,216 + GFF4176 0.25 +0.8
Or see this region's nucleotide sequence