Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3941

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3938 and GFF3939 overlap by 1 nucleotidesGFF3939 and GFF3940 are separated by 24 nucleotidesGFF3940 and GFF3941 are separated by 18 nucleotidesGFF3941 and GFF3942 overlap by 11 nucleotides GFF3938 - Ethanolamine utilization protein similar to PduL, at 220,416 to 221,048 GFF3938 GFF3939 - Propanediol utilization polyhedral body protein PduK, at 221,048 to 221,509 GFF3939 GFF3940 - Propanediol utilization polyhedral body protein PduJ, at 221,534 to 221,809 GFF3940 GFF3941 - Propanediol dehydratase reactivation factor small subunit, at 221,828 to 222,178 GFF3941 GFF3942 - Propanediol dehydratase reactivation factor large subunit, at 222,168 to 224,000 GFF3942 Position (kb) 221 222 223Strain fitness (log2 ratio) -1 0 1at 220.959 kb on - strand, within GFF3938at 221.724 kb on - strand, within GFF3940at 221.829 kb on + strandat 221.958 kb on + strand, within GFF3941at 221.958 kb on + strand, within GFF3941at 221.959 kb on - strand, within GFF3941at 222.857 kb on + strand, within GFF3942at 222.857 kb on + strand, within GFF3942at 222.858 kb on - strand, within GFF3942

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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220,959 - GFF3938 0.86 -1.2
221,724 - GFF3940 0.69 +0.4
221,829 + +0.6
221,958 + GFF3941 0.37 +1.1
221,958 + GFF3941 0.37 -1.2
221,959 - GFF3941 0.37 +0.8
222,857 + GFF3942 0.38 +0.2
222,857 + GFF3942 0.38 -0.3
222,858 - GFF3942 0.38 -0.3

Or see this region's nucleotide sequence