Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF388

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF387 and GFF388 are separated by 100 nucleotidesGFF388 and GFF389 are separated by 56 nucleotides GFF387 - Lipopolysaccharide core biosynthesis protein RfaZ, at 101,462 to 102,271 GFF387 GFF388 - Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (EC 2.4.1.56), at 102,372 to 103,517 GFF388 GFF389 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 103,574 to 104,788 GFF389 Position (kb) 102 103 104Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 101.507 kb on - strandat 101.982 kb on - strand, within GFF387at 102.025 kb on + strand, within GFF387at 102.028 kb on - strand, within GFF387at 102.641 kb on + strand, within GFF388at 102.671 kb on + strand, within GFF388at 102.671 kb on + strand, within GFF388at 102.690 kb on - strand, within GFF388at 102.759 kb on - strand, within GFF388at 103.029 kb on - strand, within GFF388at 103.148 kb on + strand, within GFF388at 103.152 kb on - strand, within GFF388at 103.196 kb on + strand, within GFF388at 103.198 kb on + strand, within GFF388at 103.355 kb on + strand, within GFF388at 103.373 kb on - strand, within GFF388at 103.553 kb on - strandat 103.555 kb on + strandat 103.764 kb on - strand, within GFF389at 103.882 kb on + strand, within GFF389at 104.016 kb on - strand, within GFF389at 104.144 kb on - strand, within GFF389at 104.197 kb on - strand, within GFF389

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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101,507 - -3.1
101,982 - GFF387 0.64 -0.6
102,025 + GFF387 0.70 -0.2
102,028 - GFF387 0.70 +0.1
102,641 + GFF388 0.23 -3.3
102,671 + GFF388 0.26 -1.0
102,671 + GFF388 0.26 -0.8
102,690 - GFF388 0.28 -1.7
102,759 - GFF388 0.34 -1.3
103,029 - GFF388 0.57 -2.0
103,148 + GFF388 0.68 -1.7
103,152 - GFF388 0.68 -1.3
103,196 + GFF388 0.72 -1.9
103,198 + GFF388 0.72 -0.1
103,355 + GFF388 0.86 -1.8
103,373 - GFF388 0.87 -1.7
103,553 - -0.2
103,555 + +0.5
103,764 - GFF389 0.16 +2.3
103,882 + GFF389 0.25 +1.8
104,016 - GFF389 0.36 +2.0
104,144 - GFF389 0.47 +2.4
104,197 - GFF389 0.51 +2.0

Or see this region's nucleotide sequence