Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF384

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF383 and GFF384 are separated by 5 nucleotidesGFF384 and GFF385 are separated by 17 nucleotides GFF383 - Lipopolysaccharide 1,6-galactosyltransferase (EC 2.4.1.-), at 97,465 to 98,544 GFF383 GFF384 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,3-glucosyltransferase WaaO (EC 2.4.1.-), at 98,550 to 99,563 GFF384 GFF385 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58), at 99,581 to 100,591 GFF385 Position (kb) 98 99 100Strain fitness (log2 ratio) -2 -1 0 1 2at 98.319 kb on + strand, within GFF383at 98.332 kb on - strand, within GFF383at 98.723 kb on - strand, within GFF384at 98.782 kb on + strand, within GFF384at 99.266 kb on + strand, within GFF384at 99.445 kb on + strand, within GFF384at 100.418 kb on - strand, within GFF385

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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98,319 + GFF383 0.79 +0.3
98,332 - GFF383 0.80 +2.4
98,723 - GFF384 0.17 -0.8
98,782 + GFF384 0.23 -1.9
99,266 + GFF384 0.71 -1.4
99,445 + GFF384 0.88 -0.8
100,418 - GFF385 0.83 -1.2

Or see this region's nucleotide sequence