Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3829 and GFF3830 are separated by 15 nucleotides GFF3830 and GFF3831 are separated by 262 nucleotides GFF3831 and GFF3832 are separated by 125 nucleotides GFF3832 and GFF3833 are separated by 98 nucleotides
GFF3829 - Fimbriae usher protein StcC, at 98,508 to 100,997
GFF3829
GFF3830 - Uncharacterized protein YehA precursor, at 101,013 to 102,020
GFF3830
GFF3831 - Uncharacterized protein YohN precursor, at 102,283 to 102,597
GFF3831
GFF3832 - FIG01045739: hypothetical protein, at 102,723 to 102,908
GFF3832
GFF3833 - Hydroxyethylthiazole kinase (EC 2.7.1.50), at 103,007 to 103,804
GFF3833
Position (kb) 101
102
103 Strain fitness (log2 ratio) -1
0
1 at 100.022 kb on - strand, within GFF3829 at 100.118 kb on + strand, within GFF3829 at 100.214 kb on + strand, within GFF3829 at 100.426 kb on - strand, within GFF3829 at 100.625 kb on + strand, within GFF3829 at 101.398 kb on + strand, within GFF3830 at 102.369 kb on - strand, within GFF3831 at 102.369 kb on - strand, within GFF3831 at 102.381 kb on + strand, within GFF3831 at 102.426 kb on + strand, within GFF3831 at 102.429 kb on + strand, within GFF3831 at 102.431 kb on + strand, within GFF3831 at 102.446 kb on + strand, within GFF3831 at 102.447 kb on - strand, within GFF3831 at 102.505 kb on + strand, within GFF3831 at 102.659 kb on + strand at 102.660 kb on - strand at 102.735 kb on - strand at 102.842 kb on + strand, within GFF3832 at 102.843 kb on - strand, within GFF3832 at 102.851 kb on + strand, within GFF3832
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 100,022 - GFF3829 0.61 -0.3 100,118 + GFF3829 0.65 +0.1 100,214 + GFF3829 0.69 +0.3 100,426 - GFF3829 0.77 +0.3 100,625 + GFF3829 0.85 +1.0 101,398 + GFF3830 0.38 -0.6 102,369 - GFF3831 0.27 -0.3 102,369 - GFF3831 0.27 +0.1 102,381 + GFF3831 0.31 -1.4 102,426 + GFF3831 0.45 +0.2 102,429 + GFF3831 0.46 -0.0 102,431 + GFF3831 0.47 -0.5 102,446 + GFF3831 0.52 +0.7 102,447 - GFF3831 0.52 -0.3 102,505 + GFF3831 0.70 -0.1 102,659 + -0.3 102,660 - +0.6 102,735 - -0.7 102,842 + GFF3832 0.64 +1.0 102,843 - GFF3832 0.65 +0.4 102,851 + GFF3832 0.69 -0.2
Or see this region's nucleotide sequence