Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF370 and GFF371 are separated by 172 nucleotides GFF371 and GFF372 are separated by 253 nucleotides GFF372 and GFF373 are separated by 20 nucleotides GFF373 and GFF374 are separated by 97 nucleotides
GFF370 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 88,337 to 89,560
GFF370
GFF371 - DNA repair protein RadC, at 89,733 to 90,398
GFF371
GFF372 - LSU ribosomal protein L28p, at 90,652 to 90,852
GFF372
GFF373 - LSU ribosomal protein L33p @ LSU ribosomal protein L33p, zinc-independent, at 90,873 to 91,040
GFF373
GFF374 - Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23), at 91,138 to 91,947
GFF374
Position (kb) 89
90
91 Strain fitness (log2 ratio) -1
0
1 at 89.714 kb on + strand at 89.829 kb on - strand, within GFF371 at 89.853 kb on - strand, within GFF371 at 89.853 kb on - strand, within GFF371 at 89.853 kb on - strand, within GFF371 at 89.853 kb on - strand, within GFF371 at 90.000 kb on + strand, within GFF371 at 90.001 kb on - strand, within GFF371 at 90.173 kb on - strand, within GFF371 at 90.273 kb on - strand, within GFF371 at 90.287 kb on + strand, within GFF371 at 91.105 kb on - strand at 91.203 kb on + strand at 91.314 kb on - strand, within GFF374 at 91.340 kb on + strand, within GFF374
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 89,714 + -1.6 89,829 - GFF371 0.14 -0.2 89,853 - GFF371 0.18 -1.0 89,853 - GFF371 0.18 +0.1 89,853 - GFF371 0.18 -0.9 89,853 - GFF371 0.18 -0.6 90,000 + GFF371 0.40 +0.3 90,001 - GFF371 0.40 -1.2 90,173 - GFF371 0.66 -0.8 90,273 - GFF371 0.81 +0.0 90,287 + GFF371 0.83 -0.7 91,105 - -0.1 91,203 + +0.3 91,314 - GFF374 0.22 -1.0 91,340 + GFF374 0.25 -0.5
Or see this region's nucleotide sequence