Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3705 and GFF3706 are separated by 276 nucleotides GFF3706 and GFF3707 are separated by 108 nucleotides GFF3707 and GFF3708 overlap by 11 nucleotides GFF3708 and GFF3709 are separated by 31 nucleotides
GFF3705 - TsgA protein homolog, at 11,689 to 12,870
GFF3705
GFF3706 - Peptidyl-prolyl cis-trans isomerase PpiA precursor (EC 5.2.1.8), at 13,147 to 13,719
GFF3706
GFF3707 - FIG00639558: hypothetical protein, at 13,828 to 13,995
GFF3707
GFF3708 - Cell filamentation protein fic, at 13,985 to 14,587
GFF3708
GFF3709 - Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85), at 14,619 to 15,182
GFF3709
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.196 kb on - strand, within GFF3705 at 12.202 kb on + strand, within GFF3705 at 12.203 kb on - strand, within GFF3705 at 12.218 kb on + strand, within GFF3705 at 12.374 kb on + strand, within GFF3705 at 12.375 kb on - strand, within GFF3705 at 12.396 kb on + strand, within GFF3705 at 12.397 kb on - strand, within GFF3705 at 12.397 kb on - strand, within GFF3705 at 12.507 kb on + strand, within GFF3705 at 12.643 kb on + strand, within GFF3705 at 12.723 kb on + strand, within GFF3705 at 12.864 kb on + strand at 12.952 kb on - strand at 13.054 kb on - strand at 13.056 kb on - strand at 13.059 kb on + strand at 13.120 kb on + strand at 13.464 kb on + strand, within GFF3706 at 13.612 kb on - strand, within GFF3706 at 13.788 kb on + strand at 13.791 kb on - strand at 13.791 kb on - strand at 13.915 kb on - strand, within GFF3707 at 13.915 kb on - strand, within GFF3707 at 13.928 kb on + strand, within GFF3707 at 13.931 kb on + strand, within GFF3707 at 13.984 kb on + strand at 14.184 kb on - strand, within GFF3708 at 14.364 kb on - strand, within GFF3708 at 14.364 kb on - strand, within GFF3708 at 14.366 kb on + strand, within GFF3708 at 14.366 kb on + strand, within GFF3708 at 14.367 kb on - strand, within GFF3708 at 14.367 kb on - strand, within GFF3708 at 14.378 kb on - strand, within GFF3708 at 14.476 kb on + strand, within GFF3708 at 14.477 kb on - strand, within GFF3708 at 14.640 kb on + strand at 14.651 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 12,196 - GFF3705 0.43 +1.3 12,202 + GFF3705 0.43 -0.0 12,203 - GFF3705 0.43 +0.2 12,218 + GFF3705 0.45 +0.1 12,374 + GFF3705 0.58 +0.4 12,375 - GFF3705 0.58 +0.6 12,396 + GFF3705 0.60 +0.3 12,397 - GFF3705 0.60 -0.1 12,397 - GFF3705 0.60 -0.0 12,507 + GFF3705 0.69 -0.6 12,643 + GFF3705 0.81 -0.1 12,723 + GFF3705 0.87 -0.0 12,864 + +0.3 12,952 - +0.6 13,054 - +0.3 13,056 - -0.2 13,059 + +0.5 13,120 + -0.2 13,464 + GFF3706 0.55 +0.5 13,612 - GFF3706 0.81 -1.1 13,788 + +0.3 13,791 - +0.7 13,791 - +0.3 13,915 - GFF3707 0.52 -0.8 13,915 - GFF3707 0.52 -0.7 13,928 + GFF3707 0.60 +0.1 13,931 + GFF3707 0.61 +0.6 13,984 + -0.1 14,184 - GFF3708 0.33 -0.1 14,364 - GFF3708 0.63 -0.3 14,364 - GFF3708 0.63 -0.1 14,366 + GFF3708 0.63 +0.3 14,366 + GFF3708 0.63 -2.2 14,367 - GFF3708 0.63 -1.3 14,367 - GFF3708 0.63 -0.0 14,378 - GFF3708 0.65 +0.6 14,476 + GFF3708 0.81 +0.5 14,477 - GFF3708 0.82 -0.9 14,640 + -0.5 14,651 + +0.1
Or see this region's nucleotide sequence