Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3540 and GFF3541 are separated by 89 nucleotides GFF3541 and GFF3542 are separated by 98 nucleotides GFF3542 and GFF3543 are separated by 56 nucleotides
GFF3540 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 292,243 to 293,883
GFF3540
GFF3541 - Ferredoxin-like protein FixX, at 293,973 to 294,164
GFF3541
GFF3542 - Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-), at 294,263 to 295,549
GFF3542
GFF3543 - Electron transfer flavoprotein, alpha subunit, at 295,606 to 296,541
GFF3543
Position (kb) 294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 293.875 kb on - strand at 293.914 kb on - strand at 294.035 kb on + strand, within GFF3541 at 294.036 kb on - strand, within GFF3541 at 294.119 kb on - strand, within GFF3541 at 294.152 kb on + strand at 294.189 kb on - strand at 294.252 kb on - strand at 294.266 kb on + strand at 294.596 kb on + strand, within GFF3542 at 294.616 kb on + strand, within GFF3542 at 294.716 kb on - strand, within GFF3542 at 294.839 kb on + strand, within GFF3542 at 294.953 kb on - strand, within GFF3542 at 295.166 kb on + strand, within GFF3542 at 295.246 kb on - strand, within GFF3542 at 295.253 kb on - strand, within GFF3542 at 295.417 kb on + strand, within GFF3542 at 295.485 kb on + strand at 295.506 kb on - strand at 295.525 kb on + strand at 295.554 kb on + strand at 295.607 kb on + strand at 295.647 kb on - strand at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.820 kb on + strand, within GFF3543 at 295.903 kb on - strand, within GFF3543 at 296.037 kb on + strand, within GFF3543 at 296.038 kb on - strand, within GFF3543 at 296.039 kb on + strand, within GFF3543 at 296.039 kb on + strand, within GFF3543 at 296.064 kb on + strand, within GFF3543 at 296.272 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.500 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 293,875 - -0.0 293,914 - +0.0 294,035 + GFF3541 0.32 +0.4 294,036 - GFF3541 0.33 +0.2 294,119 - GFF3541 0.76 +1.6 294,152 + +0.3 294,189 - -0.0 294,252 - +0.4 294,266 + -0.6 294,596 + GFF3542 0.26 +0.9 294,616 + GFF3542 0.27 +0.5 294,716 - GFF3542 0.35 +0.3 294,839 + GFF3542 0.45 -0.2 294,953 - GFF3542 0.54 +0.0 295,166 + GFF3542 0.70 +1.1 295,246 - GFF3542 0.76 +2.6 295,253 - GFF3542 0.77 -3.2 295,417 + GFF3542 0.90 -0.3 295,485 + -0.1 295,506 - -0.5 295,525 + +1.7 295,554 + +0.2 295,607 + -0.1 295,647 - -1.0 295,747 - GFF3543 0.15 -1.2 295,747 - GFF3543 0.15 -0.4 295,747 - GFF3543 0.15 -3.2 295,747 - GFF3543 0.15 -0.2 295,820 + GFF3543 0.23 -0.3 295,903 - GFF3543 0.32 +0.1 296,037 + GFF3543 0.46 +1.2 296,038 - GFF3543 0.46 +0.5 296,039 + GFF3543 0.46 +0.4 296,039 + GFF3543 0.46 +2.0 296,064 + GFF3543 0.49 -0.4 296,272 - GFF3543 0.71 -0.3 296,387 - GFF3543 0.83 -0.1 296,387 - GFF3543 0.83 +0.4 296,387 - GFF3543 0.83 -0.3 296,500 + -0.9
Or see this region's nucleotide sequence