Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3346 and GFF3347 are separated by 35 nucleotides GFF3347 and GFF3348 are separated by 1 nucleotides GFF3348 and GFF3349 are separated by 264 nucleotides
GFF3346 - Ribosomal-protein-S5p-alanine acetyltransferase, at 114,558 to 115,142
GFF3346
GFF3347 - Protein of unknown function YceH, at 115,178 to 115,825
GFF3347
GFF3348 - Virulence factor MviM, at 115,827 to 116,750
GFF3348
GFF3349 - Proposed peptidoglycan lipid II flippase MurJ, at 117,015 to 118,589
GFF3349
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-1
0
1
2
3 at 114.948 kb on + strand, within GFF3346 at 114.949 kb on - strand, within GFF3346 at 114.969 kb on - strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 115.291 kb on + strand, within GFF3347 at 115.292 kb on - strand, within GFF3347 at 115.490 kb on - strand, within GFF3347 at 115.665 kb on - strand, within GFF3347 at 115.667 kb on - strand, within GFF3347 at 115.783 kb on - strand at 115.806 kb on + strand at 115.894 kb on + strand at 115.946 kb on + strand, within GFF3348 at 115.954 kb on + strand, within GFF3348 at 115.955 kb on - strand, within GFF3348 at 115.979 kb on - strand, within GFF3348 at 115.983 kb on + strand, within GFF3348 at 115.984 kb on - strand, within GFF3348 at 116.042 kb on + strand, within GFF3348 at 116.251 kb on + strand, within GFF3348 at 116.251 kb on + strand, within GFF3348 at 116.255 kb on + strand, within GFF3348 at 116.256 kb on - strand, within GFF3348 at 116.256 kb on - strand, within GFF3348 at 116.258 kb on - strand, within GFF3348 at 116.271 kb on - strand, within GFF3348 at 116.320 kb on - strand, within GFF3348 at 116.453 kb on + strand, within GFF3348 at 116.462 kb on + strand, within GFF3348 at 116.463 kb on - strand, within GFF3348 at 116.521 kb on + strand, within GFF3348 at 116.522 kb on - strand, within GFF3348 at 116.591 kb on + strand, within GFF3348 at 116.592 kb on - strand, within GFF3348 at 116.749 kb on - strand at 116.793 kb on + strand at 116.796 kb on + strand at 116.796 kb on + strand at 116.797 kb on - strand at 116.798 kb on + strand at 116.858 kb on + strand at 116.860 kb on + strand at 116.862 kb on + strand at 116.862 kb on + strand at 116.862 kb on + strand at 116.862 kb on + strand at 116.862 kb on + strand at 116.880 kb on + strand at 116.880 kb on + strand at 116.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 114,948 + GFF3346 0.67 +0.3 114,949 - GFF3346 0.67 -0.4 114,969 - GFF3346 0.70 +0.9 114,990 + GFF3346 0.74 +1.1 114,990 + GFF3346 0.74 +0.2 115,291 + GFF3347 0.17 +0.2 115,292 - GFF3347 0.18 -0.9 115,490 - GFF3347 0.48 -0.7 115,665 - GFF3347 0.75 +0.3 115,667 - GFF3347 0.75 -0.1 115,783 - -0.2 115,806 + -0.5 115,894 + +0.6 115,946 + GFF3348 0.13 +1.2 115,954 + GFF3348 0.14 +0.2 115,955 - GFF3348 0.14 -0.4 115,979 - GFF3348 0.16 -0.4 115,983 + GFF3348 0.17 -1.0 115,984 - GFF3348 0.17 +1.2 116,042 + GFF3348 0.23 +0.9 116,251 + GFF3348 0.46 -0.4 116,251 + GFF3348 0.46 +0.4 116,255 + GFF3348 0.46 +0.3 116,256 - GFF3348 0.46 +0.4 116,256 - GFF3348 0.46 +2.8 116,258 - GFF3348 0.47 +0.8 116,271 - GFF3348 0.48 -0.5 116,320 - GFF3348 0.53 +0.6 116,453 + GFF3348 0.68 +0.4 116,462 + GFF3348 0.69 -0.4 116,463 - GFF3348 0.69 +0.6 116,521 + GFF3348 0.75 +0.2 116,522 - GFF3348 0.75 +0.5 116,591 + GFF3348 0.83 +2.2 116,592 - GFF3348 0.83 -0.5 116,749 - +0.8 116,793 + +1.0 116,796 + +0.1 116,796 + +0.3 116,797 - +0.4 116,798 + -0.4 116,858 + -1.6 116,860 + -0.3 116,862 + +0.5 116,862 + -1.7 116,862 + +0.6 116,862 + +0.1 116,862 + -1.3 116,880 + -0.4 116,880 + +0.1 116,943 + +0.4
Or see this region's nucleotide sequence