Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3348

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3346 and GFF3347 are separated by 35 nucleotidesGFF3347 and GFF3348 are separated by 1 nucleotidesGFF3348 and GFF3349 are separated by 264 nucleotides GFF3346 - Ribosomal-protein-S5p-alanine acetyltransferase, at 114,558 to 115,142 GFF3346 GFF3347 - Protein of unknown function YceH, at 115,178 to 115,825 GFF3347 GFF3348 - Virulence factor MviM, at 115,827 to 116,750 GFF3348 GFF3349 - Proposed peptidoglycan lipid II flippase MurJ, at 117,015 to 118,589 GFF3349 Position (kb) 115 116 117Strain fitness (log2 ratio) -1 0 1 2 3at 114.948 kb on + strand, within GFF3346at 114.949 kb on - strand, within GFF3346at 114.969 kb on - strand, within GFF3346at 114.990 kb on + strand, within GFF3346at 114.990 kb on + strand, within GFF3346at 115.291 kb on + strand, within GFF3347at 115.292 kb on - strand, within GFF3347at 115.490 kb on - strand, within GFF3347at 115.665 kb on - strand, within GFF3347at 115.667 kb on - strand, within GFF3347at 115.783 kb on - strandat 115.806 kb on + strandat 115.894 kb on + strandat 115.946 kb on + strand, within GFF3348at 115.954 kb on + strand, within GFF3348at 115.955 kb on - strand, within GFF3348at 115.979 kb on - strand, within GFF3348at 115.983 kb on + strand, within GFF3348at 115.984 kb on - strand, within GFF3348at 116.042 kb on + strand, within GFF3348at 116.251 kb on + strand, within GFF3348at 116.251 kb on + strand, within GFF3348at 116.255 kb on + strand, within GFF3348at 116.256 kb on - strand, within GFF3348at 116.256 kb on - strand, within GFF3348at 116.258 kb on - strand, within GFF3348at 116.271 kb on - strand, within GFF3348at 116.320 kb on - strand, within GFF3348at 116.453 kb on + strand, within GFF3348at 116.462 kb on + strand, within GFF3348at 116.463 kb on - strand, within GFF3348at 116.521 kb on + strand, within GFF3348at 116.522 kb on - strand, within GFF3348at 116.591 kb on + strand, within GFF3348at 116.592 kb on - strand, within GFF3348at 116.749 kb on - strandat 116.793 kb on + strandat 116.796 kb on + strandat 116.796 kb on + strandat 116.797 kb on - strandat 116.798 kb on + strandat 116.858 kb on + strandat 116.860 kb on + strandat 116.862 kb on + strandat 116.862 kb on + strandat 116.862 kb on + strandat 116.862 kb on + strandat 116.862 kb on + strandat 116.880 kb on + strandat 116.880 kb on + strandat 116.943 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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114,948 + GFF3346 0.67 +0.3
114,949 - GFF3346 0.67 -0.4
114,969 - GFF3346 0.70 +0.9
114,990 + GFF3346 0.74 +1.1
114,990 + GFF3346 0.74 +0.2
115,291 + GFF3347 0.17 +0.2
115,292 - GFF3347 0.18 -0.9
115,490 - GFF3347 0.48 -0.7
115,665 - GFF3347 0.75 +0.3
115,667 - GFF3347 0.75 -0.1
115,783 - -0.2
115,806 + -0.5
115,894 + +0.6
115,946 + GFF3348 0.13 +1.2
115,954 + GFF3348 0.14 +0.2
115,955 - GFF3348 0.14 -0.4
115,979 - GFF3348 0.16 -0.4
115,983 + GFF3348 0.17 -1.0
115,984 - GFF3348 0.17 +1.2
116,042 + GFF3348 0.23 +0.9
116,251 + GFF3348 0.46 -0.4
116,251 + GFF3348 0.46 +0.4
116,255 + GFF3348 0.46 +0.3
116,256 - GFF3348 0.46 +0.4
116,256 - GFF3348 0.46 +2.8
116,258 - GFF3348 0.47 +0.8
116,271 - GFF3348 0.48 -0.5
116,320 - GFF3348 0.53 +0.6
116,453 + GFF3348 0.68 +0.4
116,462 + GFF3348 0.69 -0.4
116,463 - GFF3348 0.69 +0.6
116,521 + GFF3348 0.75 +0.2
116,522 - GFF3348 0.75 +0.5
116,591 + GFF3348 0.83 +2.2
116,592 - GFF3348 0.83 -0.5
116,749 - +0.8
116,793 + +1.0
116,796 + +0.1
116,796 + +0.3
116,797 - +0.4
116,798 + -0.4
116,858 + -1.6
116,860 + -0.3
116,862 + +0.5
116,862 + -1.7
116,862 + +0.6
116,862 + +0.1
116,862 + -1.3
116,880 + -0.4
116,880 + +0.1
116,943 + +0.4

Or see this region's nucleotide sequence