Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3229

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3228 and GFF3229 are separated by 475 nucleotidesGFF3229 and GFF3230 are separated by 267 nucleotidesGFF3230 and GFF3231 are separated by 131 nucleotides GFF3228 - Phage tail fibers, at 780 to 1,361 GFF3228 GFF3229 - Secreted effector protein, at 1,837 to 2,805 GFF3229 GFF3230 - Transposase, at 3,073 to 3,321 GFF3230 GFF3231 - Gifsy-2 prophage protein, at 3,453 to 4,079 GFF3231 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1 2at 0.839 kb on + strand, within GFF3228at 0.871 kb on - strand, within GFF3228at 0.872 kb on + strand, within GFF3228at 0.872 kb on + strand, within GFF3228at 0.947 kb on + strand, within GFF3228at 0.948 kb on - strand, within GFF3228at 1.035 kb on + strand, within GFF3228at 1.035 kb on + strand, within GFF3228at 1.112 kb on + strand, within GFF3228at 1.295 kb on + strand, within GFF3228at 1.307 kb on - strandat 1.440 kb on - strandat 1.451 kb on + strandat 1.451 kb on + strandat 1.481 kb on + strandat 1.482 kb on - strandat 1.809 kb on - strandat 1.809 kb on - strandat 2.229 kb on - strand, within GFF3229at 2.553 kb on + strand, within GFF3229at 2.881 kb on + strandat 3.021 kb on - strandat 3.186 kb on + strand, within GFF3230at 3.351 kb on + strandat 3.455 kb on - strandat 3.455 kb on - strandat 3.635 kb on + strand, within GFF3231

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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839 + GFF3228 0.10 +0.2
871 - GFF3228 0.16 +1.2
872 + GFF3228 0.16 +0.1
872 + GFF3228 0.16 -0.2
947 + GFF3228 0.29 -0.4
948 - GFF3228 0.29 -0.7
1,035 + GFF3228 0.44 +0.1
1,035 + GFF3228 0.44 -0.2
1,112 + GFF3228 0.57 +0.6
1,295 + GFF3228 0.88 -0.1
1,307 - +0.7
1,440 - -0.1
1,451 + +0.3
1,451 + -0.2
1,481 + +0.5
1,482 - +0.2
1,809 - +1.8
1,809 - +0.8
2,229 - GFF3229 0.40 -1.0
2,553 + GFF3229 0.74 -0.6
2,881 + +0.1
3,021 - -0.3
3,186 + GFF3230 0.45 +0.4
3,351 + +0.1
3,455 - -0.8
3,455 - -0.1
3,635 + GFF3231 0.29 -0.2

Or see this region's nucleotide sequence