Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF32

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF29 and GFF30 overlap by 4 nucleotidesGFF30 and GFF31 are separated by 197 nucleotidesGFF31 and GFF32 are separated by 52 nucleotidesGFF32 and GFF33 are separated by 83 nucleotides GFF29 - Hypothetical protein YaeJ with similarity to translation release factor, at 7,433 to 7,855 GFF29 GFF30 - YaeQ protein, at 7,852 to 8,397 GFF30 GFF31 - FIG00639395: hypothetical protein, at 8,595 to 8,795 GFF31 GFF32 - Rho-specific inhibitor of transcription termination (YaeO), at 8,848 to 9,042 GFF32 GFF33 - tRNA(Ile)-lysidine synthetase (EC 6.3.4.19), at 9,126 to 10,418 GFF33 Position (kb) 8 9 10Strain fitness (log2 ratio) -1 0 1at 8.078 kb on + strand, within GFF30at 8.250 kb on - strand, within GFF30at 8.373 kb on + strandat 8.445 kb on + strandat 8.511 kb on + strandat 8.512 kb on - strandat 8.512 kb on - strandat 8.513 kb on + strandat 8.513 kb on + strandat 8.513 kb on + strandat 8.513 kb on + strandat 8.513 kb on + strandat 8.514 kb on - strandat 8.514 kb on - strandat 8.526 kb on - strandat 8.551 kb on - strandat 8.604 kb on + strandat 8.617 kb on + strand, within GFF31at 8.653 kb on + strand, within GFF31at 8.658 kb on + strand, within GFF31at 8.696 kb on + strand, within GFF31at 8.781 kb on + strandat 8.800 kb on + strandat 8.825 kb on - strandat 8.829 kb on + strandat 8.830 kb on - strandat 8.859 kb on - strandat 8.938 kb on + strand, within GFF32

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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8,078 + GFF30 0.41 -0.4
8,250 - GFF30 0.73 -0.3
8,373 + +0.1
8,445 + -0.7
8,511 + -0.2
8,512 - -0.9
8,512 - -1.5
8,513 + +0.4
8,513 + +0.2
8,513 + -0.5
8,513 + +0.2
8,513 + -0.1
8,514 - +0.2
8,514 - -0.3
8,526 - +0.7
8,551 - -0.3
8,604 + -0.1
8,617 + GFF31 0.11 -0.6
8,653 + GFF31 0.29 -0.1
8,658 + GFF31 0.31 -0.9
8,696 + GFF31 0.50 +0.2
8,781 + +0.0
8,800 + +0.1
8,825 - -0.8
8,829 + +0.3
8,830 - +0.1
8,859 - +0.2
8,938 + GFF32 0.46 -0.7

Or see this region's nucleotide sequence