Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3165 and GFF3166 are separated by 459 nucleotides GFF3166 and GFF3167 are separated by 47 nucleotides
GFF3165 - Inner membrane protein YqiK, at 165,555 to 167,234
GFF3165
GFF3166 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 167,694 to 169,127
GFF3166
GFF3167 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 169,175 to 172,018
GFF3167
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 166.912 kb on + strand, within GFF3165 at 166.921 kb on + strand, within GFF3165 at 167.095 kb on - strand at 167.095 kb on - strand at 167.245 kb on + strand at 167.246 kb on - strand at 167.509 kb on - strand at 167.573 kb on - strand at 167.695 kb on + strand at 167.940 kb on - strand, within GFF3166 at 167.987 kb on + strand, within GFF3166 at 168.250 kb on - strand, within GFF3166 at 168.358 kb on + strand, within GFF3166 at 168.359 kb on - strand, within GFF3166 at 168.383 kb on + strand, within GFF3166 at 168.719 kb on + strand, within GFF3166 at 168.798 kb on + strand, within GFF3166 at 168.799 kb on - strand, within GFF3166 at 168.802 kb on - strand, within GFF3166 at 169.051 kb on + strand at 169.072 kb on - strand at 169.167 kb on - strand at 169.167 kb on - strand at 169.172 kb on - strand at 169.172 kb on - strand at 169.172 kb on - strand at 169.232 kb on - strand at 169.306 kb on - strand at 169.343 kb on - strand at 169.462 kb on + strand, within GFF3167 at 169.463 kb on - strand, within GFF3167 at 169.618 kb on + strand, within GFF3167 at 169.635 kb on + strand, within GFF3167 at 169.636 kb on - strand, within GFF3167 at 169.637 kb on + strand, within GFF3167 at 169.638 kb on - strand, within GFF3167 at 169.751 kb on + strand, within GFF3167 at 169.834 kb on - strand, within GFF3167 at 169.923 kb on + strand, within GFF3167
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 166,912 + GFF3165 0.81 +0.7 166,921 + GFF3165 0.81 -0.1 167,095 - -0.2 167,095 - +0.0 167,245 + -0.3 167,246 - -0.5 167,509 - -0.7 167,573 - +0.5 167,695 + +0.8 167,940 - GFF3166 0.17 -0.9 167,987 + GFF3166 0.20 -1.1 168,250 - GFF3166 0.39 -0.7 168,358 + GFF3166 0.46 -0.8 168,359 - GFF3166 0.46 +0.2 168,383 + GFF3166 0.48 +1.3 168,719 + GFF3166 0.71 -0.8 168,798 + GFF3166 0.77 +1.5 168,799 - GFF3166 0.77 -3.4 168,802 - GFF3166 0.77 -0.9 169,051 + -0.4 169,072 - +0.2 169,167 - +0.7 169,167 - -1.1 169,172 - -0.6 169,172 - -0.0 169,172 - +0.0 169,232 - -0.2 169,306 - +0.8 169,343 - +0.2 169,462 + GFF3167 0.10 -0.8 169,463 - GFF3167 0.10 +0.2 169,618 + GFF3167 0.16 -0.2 169,635 + GFF3167 0.16 +0.4 169,636 - GFF3167 0.16 +0.1 169,637 + GFF3167 0.16 -0.3 169,638 - GFF3167 0.16 +0.5 169,751 + GFF3167 0.20 -0.4 169,834 - GFF3167 0.23 -0.4 169,923 + GFF3167 0.26 +0.6
Or see this region's nucleotide sequence