Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3072 and GFF3214 are separated by 105 nucleotides GFF3214 and GFF3073 are separated by 603 nucleotides GFF3073 and GFF3074 are separated by 24 nucleotides GFF3074 and GFF3075 are separated by 23 nucleotides
GFF3072 - Putative inner membrane protein YqgA, at 80,468 to 81,175
GFF3072
GFF3214 - tRNA-Phe-GAA, at 81,281 to 81,356
GFF3214
GFF3073 - 4-hydroxybutyrate:acetyl-CoA CoA transferase (EC 2.3.1.-), at 81,960 to 83,285
GFF3073
GFF3074 - Acyl dehydratase, at 83,310 to 83,837
GFF3074
GFF3075 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 83,861 to 84,703
GFF3075
Position (kb) 81
82
83
84 Strain fitness (log2 ratio) -1
0
1
2
3 at 80.974 kb on - strand, within GFF3072 at 81.081 kb on + strand, within GFF3072 at 81.113 kb on - strand at 81.188 kb on + strand at 81.220 kb on - strand at 81.261 kb on - strand at 81.488 kb on + strand at 81.564 kb on + strand at 81.933 kb on - strand at 81.987 kb on + strand at 82.122 kb on + strand, within GFF3073 at 82.122 kb on + strand, within GFF3073 at 82.122 kb on + strand, within GFF3073 at 82.123 kb on - strand, within GFF3073 at 82.123 kb on - strand, within GFF3073 at 82.148 kb on + strand, within GFF3073 at 82.277 kb on - strand, within GFF3073 at 82.421 kb on - strand, within GFF3073 at 82.421 kb on - strand, within GFF3073 at 82.425 kb on - strand, within GFF3073 at 82.800 kb on - strand, within GFF3073 at 82.871 kb on + strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 83.027 kb on + strand, within GFF3073 at 83.027 kb on + strand, within GFF3073 at 83.199 kb on - strand at 83.223 kb on + strand at 83.264 kb on - strand at 83.356 kb on - strand at 83.403 kb on - strand, within GFF3074 at 83.403 kb on - strand, within GFF3074 at 83.482 kb on + strand, within GFF3074 at 83.489 kb on + strand, within GFF3074 at 83.490 kb on - strand, within GFF3074 at 83.564 kb on + strand, within GFF3074 at 83.922 kb on - strand at 84.023 kb on + strand, within GFF3075
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 80,974 - GFF3072 0.71 -0.1 81,081 + GFF3072 0.87 -0.2 81,113 - -0.7 81,188 + -1.2 81,220 - -0.1 81,261 - -0.2 81,488 + -0.0 81,564 + -0.4 81,933 - +0.1 81,987 + -0.6 82,122 + GFF3073 0.12 +0.2 82,122 + GFF3073 0.12 -0.5 82,122 + GFF3073 0.12 +0.1 82,123 - GFF3073 0.12 -0.5 82,123 - GFF3073 0.12 +1.0 82,148 + GFF3073 0.14 +0.1 82,277 - GFF3073 0.24 +0.1 82,421 - GFF3073 0.35 +3.1 82,421 - GFF3073 0.35 +0.9 82,425 - GFF3073 0.35 +0.5 82,800 - GFF3073 0.63 -0.1 82,871 + GFF3073 0.69 +0.4 82,961 - GFF3073 0.75 +0.6 82,961 - GFF3073 0.75 -0.1 82,961 - GFF3073 0.75 +0.1 83,027 + GFF3073 0.80 +0.9 83,027 + GFF3073 0.80 -0.7 83,199 - +0.1 83,223 + -0.5 83,264 - +0.3 83,356 - +0.7 83,403 - GFF3074 0.18 -0.0 83,403 - GFF3074 0.18 -0.3 83,482 + GFF3074 0.33 -0.8 83,489 + GFF3074 0.34 -0.2 83,490 - GFF3074 0.34 -0.0 83,564 + GFF3074 0.48 -1.4 83,922 - -0.1 84,023 + GFF3075 0.19 -0.3
Or see this region's nucleotide sequence