Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2944 and GFF2945 are separated by 6 nucleotides GFF2945 and GFF2946 are separated by 47 nucleotides GFF2946 and GFF2947 are separated by 22 nucleotides GFF2947 and GFF2948 are separated by 117 nucleotides GFF2948 and GFF2949 are separated by 45 nucleotides
GFF2944 - LptA, protein essential for LPS transport across the periplasm, at 24,552 to 25,106
GFF2944
GFF2945 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 25,113 to 25,838
GFF2945
GFF2946 - RNA polymerase sigma-54 factor RpoN, at 25,886 to 27,319
GFF2946
GFF2947 - Ribosome hibernation protein YhbH, at 27,342 to 27,629
GFF2947
GFF2948 - PTS system nitrogen-specific IIA component, PtsN, at 27,747 to 28,238
GFF2948
GFF2949 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 28,284 to 29,138
GFF2949
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 25.863 kb on - strand at 25.941 kb on + strand at 26.197 kb on + strand, within GFF2946 at 26.219 kb on + strand, within GFF2946 at 26.543 kb on - strand, within GFF2946 at 26.543 kb on - strand, within GFF2946 at 26.696 kb on + strand, within GFF2946 at 26.697 kb on - strand, within GFF2946 at 26.728 kb on + strand, within GFF2946 at 26.753 kb on - strand, within GFF2946 at 26.753 kb on - strand, within GFF2946 at 26.765 kb on - strand, within GFF2946 at 26.783 kb on - strand, within GFF2946 at 26.793 kb on - strand, within GFF2946 at 26.854 kb on - strand, within GFF2946 at 26.966 kb on + strand, within GFF2946 at 26.966 kb on + strand, within GFF2946 at 26.969 kb on - strand, within GFF2946 at 27.236 kb on - strand at 27.353 kb on + strand at 27.365 kb on + strand at 27.377 kb on + strand, within GFF2947 at 27.452 kb on - strand, within GFF2947 at 27.453 kb on + strand, within GFF2947 at 27.453 kb on + strand, within GFF2947 at 27.454 kb on - strand, within GFF2947 at 27.503 kb on - strand, within GFF2947 at 27.548 kb on + strand, within GFF2947 at 27.549 kb on - strand, within GFF2947 at 27.552 kb on + strand, within GFF2947 at 27.552 kb on + strand, within GFF2947 at 27.553 kb on - strand, within GFF2947 at 27.553 kb on - strand, within GFF2947 at 27.688 kb on + strand at 27.688 kb on + strand at 27.747 kb on - strand at 27.752 kb on - strand at 27.801 kb on - strand, within GFF2948 at 27.945 kb on - strand, within GFF2948 at 27.989 kb on - strand, within GFF2948 at 27.989 kb on - strand, within GFF2948 at 28.194 kb on + strand at 28.236 kb on + strand at 28.281 kb on + strand at 28.289 kb on - strand at 28.289 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 25,863 - +0.3 25,941 + -0.8 26,197 + GFF2946 0.22 -0.8 26,219 + GFF2946 0.23 -0.2 26,543 - GFF2946 0.46 -0.4 26,543 - GFF2946 0.46 +0.2 26,696 + GFF2946 0.56 -2.6 26,697 - GFF2946 0.57 -0.3 26,728 + GFF2946 0.59 -0.5 26,753 - GFF2946 0.60 +0.2 26,753 - GFF2946 0.60 -1.4 26,765 - GFF2946 0.61 +0.6 26,783 - GFF2946 0.63 -1.4 26,793 - GFF2946 0.63 -1.4 26,854 - GFF2946 0.68 +1.2 26,966 + GFF2946 0.75 -0.3 26,966 + GFF2946 0.75 -0.9 26,969 - GFF2946 0.76 +0.6 27,236 - -1.4 27,353 + +1.9 27,365 + +0.5 27,377 + GFF2947 0.12 +0.2 27,452 - GFF2947 0.38 -0.7 27,453 + GFF2947 0.39 -0.1 27,453 + GFF2947 0.39 +0.3 27,454 - GFF2947 0.39 +0.2 27,503 - GFF2947 0.56 -0.1 27,548 + GFF2947 0.72 -0.7 27,549 - GFF2947 0.72 -0.5 27,552 + GFF2947 0.73 -1.0 27,552 + GFF2947 0.73 +0.2 27,553 - GFF2947 0.73 +0.4 27,553 - GFF2947 0.73 -0.1 27,688 + +0.1 27,688 + -0.1 27,747 - -0.2 27,752 - +0.4 27,801 - GFF2948 0.11 -0.9 27,945 - GFF2948 0.40 +0.5 27,989 - GFF2948 0.49 -0.4 27,989 - GFF2948 0.49 -0.9 28,194 + +0.2 28,236 + +1.1 28,281 + +2.0 28,289 - -1.4 28,289 - -1.1
Or see this region's nucleotide sequence