Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2921

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2919 and GFF2920 are separated by 103 nucleotidesGFF2920 and GFF2921 are separated by 87 nucleotidesGFF2921 and GFF2922 are separated by 155 nucleotidesGFF2922 and GFF2923 are separated by 53 nucleotidesGFF2923 and GFF2924 are separated by 65 nucleotides GFF2919 - Cell division protein FtsH (EC 3.4.24.-), at 6,166 to 8,100 GFF2919 GFF2920 - Cell division protein FtsJ / Ribosomal RNA large subunit methyltransferase E (EC 2.1.1.-) ## LSU rRNA Um2552, at 8,204 to 8,830 GFF2920 GFF2921 - FIG004454: RNA binding protein, at 8,918 to 9,250 GFF2921 GFF2922 - Transcription elongation factor GreA, at 9,406 to 9,882 GFF2922 GFF2923 - hypothetical protein, at 9,936 to 10,064 GFF2923 GFF2924 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 10,130 to 11,563 GFF2924 Position (kb) 8 9 10Strain fitness (log2 ratio) -1 0 1at 8.182 kb on + strandat 8.888 kb on - strandat 8.988 kb on + strand, within GFF2921at 8.989 kb on - strand, within GFF2921at 9.079 kb on - strand, within GFF2921at 9.220 kb on + strandat 9.220 kb on + strandat 9.371 kb on + strandat 9.376 kb on + strandat 9.394 kb on - strandat 9.740 kb on + strand, within GFF2922at 10.015 kb on - strand, within GFF2923at 10.092 kb on - strandat 10.094 kb on + strandat 10.095 kb on - strandat 10.095 kb on - strandat 10.095 kb on - strandat 10.169 kb on - strandat 10.171 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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8,182 + +0.7
8,888 - -0.1
8,988 + GFF2921 0.21 -0.8
8,989 - GFF2921 0.21 -1.3
9,079 - GFF2921 0.48 -0.5
9,220 + +0.3
9,220 + -0.7
9,371 + -0.2
9,376 + -0.0
9,394 - +0.1
9,740 + GFF2922 0.70 +0.5
10,015 - GFF2923 0.61 +1.1
10,092 - -1.4
10,094 + +0.8
10,095 - +0.4
10,095 - -0.3
10,095 - +0.2
10,169 - -0.0
10,171 - +0.0

Or see this region's nucleotide sequence