Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2717 and GFF2718 overlap by 4 nucleotides GFF2718 and GFF2719 are separated by 86 nucleotides GFF2719 and GFF2720 are separated by 100 nucleotides GFF2720 and GFF2721 are separated by 7 nucleotides
GFF2717 - YciK-like oxidoreductase, at 239,987 to 240,748
GFF2717
GFF2718 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 240,745 to 241,335
GFF2718
GFF2719 - Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70), at 241,422 to 242,297
GFF2719
GFF2720 - Hypothetical YciO protein, TsaC/YrdC paralog, at 242,398 to 243,018
GFF2720
GFF2721 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 243,026 to 243,874
GFF2721
Position (kb) 241
242
243 Strain fitness (log2 ratio) -1
0
1
2 at 240.433 kb on + strand, within GFF2717 at 240.480 kb on - strand, within GFF2717 at 240.782 kb on + strand at 240.834 kb on + strand, within GFF2718 at 240.859 kb on - strand, within GFF2718 at 240.936 kb on + strand, within GFF2718 at 241.059 kb on + strand, within GFF2718 at 241.110 kb on + strand, within GFF2718 at 241.135 kb on + strand, within GFF2718 at 241.136 kb on - strand, within GFF2718 at 241.136 kb on - strand, within GFF2718 at 241.147 kb on + strand, within GFF2718 at 241.153 kb on + strand, within GFF2718 at 241.154 kb on - strand, within GFF2718 at 241.157 kb on + strand, within GFF2718 at 241.216 kb on - strand, within GFF2718 at 241.219 kb on - strand, within GFF2718 at 241.330 kb on + strand at 241.406 kb on + strand at 241.407 kb on - strand at 241.465 kb on - strand at 241.465 kb on - strand at 241.583 kb on - strand, within GFF2719 at 241.748 kb on - strand, within GFF2719 at 241.790 kb on + strand, within GFF2719 at 241.791 kb on - strand, within GFF2719 at 241.902 kb on - strand, within GFF2719 at 242.081 kb on - strand, within GFF2719 at 242.297 kb on + strand at 242.399 kb on + strand at 242.402 kb on - strand at 242.671 kb on - strand, within GFF2720 at 242.730 kb on - strand, within GFF2720 at 242.781 kb on - strand, within GFF2720 at 242.787 kb on - strand, within GFF2720 at 242.932 kb on - strand, within GFF2720 at 242.932 kb on - strand, within GFF2720 at 243.001 kb on - strand at 243.003 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 240,433 + GFF2717 0.59 +1.1 240,480 - GFF2717 0.65 -0.0 240,782 + +0.1 240,834 + GFF2718 0.15 -0.4 240,859 - GFF2718 0.19 -0.6 240,936 + GFF2718 0.32 -0.1 241,059 + GFF2718 0.53 +0.3 241,110 + GFF2718 0.62 -0.4 241,135 + GFF2718 0.66 -1.1 241,136 - GFF2718 0.66 +1.1 241,136 - GFF2718 0.66 -1.1 241,147 + GFF2718 0.68 -0.2 241,153 + GFF2718 0.69 +0.6 241,154 - GFF2718 0.69 -0.5 241,157 + GFF2718 0.70 -0.1 241,216 - GFF2718 0.80 -0.4 241,219 - GFF2718 0.80 +0.5 241,330 + +0.1 241,406 + -0.1 241,407 - +0.2 241,465 - -0.1 241,465 - +0.5 241,583 - GFF2719 0.18 -0.9 241,748 - GFF2719 0.37 -1.5 241,790 + GFF2719 0.42 -0.7 241,791 - GFF2719 0.42 +0.7 241,902 - GFF2719 0.55 -0.3 242,081 - GFF2719 0.75 -0.6 242,297 + +0.7 242,399 + +2.7 242,402 - -1.3 242,671 - GFF2720 0.44 -0.1 242,730 - GFF2720 0.53 +0.2 242,781 - GFF2720 0.62 +0.9 242,787 - GFF2720 0.63 +0.3 242,932 - GFF2720 0.86 +0.1 242,932 - GFF2720 0.86 +0.1 243,001 - +0.0 243,003 - +0.2
Or see this region's nucleotide sequence