Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2697 and GFF2698 are separated by 241 nucleotides GFF2698 and GFF2699 are separated by 252 nucleotides
GFF2697 - FIG01220641: hypothetical protein, at 218,742 to 219,722
GFF2697
GFF2698 - Exoribonuclease II (EC 3.1.13.1), at 219,964 to 221,898
GFF2698
GFF2699 - FIG00637999: hypothetical protein, at 222,151 to 224,142
GFF2699
Position (kb) 219
220
221
222 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.975 kb on + strand, within GFF2697 at 218.975 kb on + strand, within GFF2697 at 218.981 kb on + strand, within GFF2697 at 218.996 kb on + strand, within GFF2697 at 218.996 kb on + strand, within GFF2697 at 218.997 kb on - strand, within GFF2697 at 219.025 kb on + strand, within GFF2697 at 219.054 kb on + strand, within GFF2697 at 219.223 kb on + strand, within GFF2697 at 219.224 kb on - strand, within GFF2697 at 219.526 kb on + strand, within GFF2697 at 219.561 kb on - strand, within GFF2697 at 219.582 kb on - strand, within GFF2697 at 219.656 kb on - strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.807 kb on - strand at 219.807 kb on - strand at 219.838 kb on + strand at 219.871 kb on - strand at 219.955 kb on + strand at 220.411 kb on + strand, within GFF2698 at 220.412 kb on - strand, within GFF2698 at 220.601 kb on - strand, within GFF2698 at 220.601 kb on - strand, within GFF2698 at 220.681 kb on - strand, within GFF2698 at 220.720 kb on + strand, within GFF2698 at 221.129 kb on + strand, within GFF2698 at 221.207 kb on + strand, within GFF2698 at 221.211 kb on + strand, within GFF2698 at 221.463 kb on + strand, within GFF2698 at 221.541 kb on + strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.609 kb on + strand, within GFF2698 at 221.611 kb on + strand, within GFF2698 at 221.611 kb on + strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.646 kb on + strand, within GFF2698 at 221.688 kb on + strand, within GFF2698 at 221.787 kb on + strand at 221.787 kb on + strand at 222.056 kb on + strand at 222.062 kb on + strand at 222.104 kb on + strand at 222.156 kb on - strand at 222.241 kb on + strand at 222.242 kb on - strand at 222.287 kb on + strand at 222.396 kb on + strand, within GFF2699 at 222.396 kb on + strand, within GFF2699 at 222.500 kb on + strand, within GFF2699 at 222.664 kb on + strand, within GFF2699
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 218,975 + GFF2697 0.24 -1.1 218,975 + GFF2697 0.24 +2.3 218,981 + GFF2697 0.24 -0.1 218,996 + GFF2697 0.26 +0.0 218,996 + GFF2697 0.26 +1.0 218,997 - GFF2697 0.26 +0.5 219,025 + GFF2697 0.29 -1.5 219,054 + GFF2697 0.32 -0.1 219,223 + GFF2697 0.49 +0.3 219,224 - GFF2697 0.49 -0.9 219,526 + GFF2697 0.80 -0.5 219,561 - GFF2697 0.83 -1.0 219,582 - GFF2697 0.86 -0.0 219,656 - +1.0 219,806 + +0.1 219,806 + -0.3 219,806 + +0.4 219,806 + +1.5 219,806 + +0.4 219,807 - +0.8 219,807 - +0.3 219,838 + -0.1 219,871 - +0.2 219,955 + +0.1 220,411 + GFF2698 0.23 -0.9 220,412 - GFF2698 0.23 -1.2 220,601 - GFF2698 0.33 -0.8 220,601 - GFF2698 0.33 -3.2 220,681 - GFF2698 0.37 -0.4 220,720 + GFF2698 0.39 -1.6 221,129 + GFF2698 0.60 -0.6 221,207 + GFF2698 0.64 -0.9 221,211 + GFF2698 0.64 -0.4 221,463 + GFF2698 0.77 -1.1 221,541 + GFF2698 0.81 -2.3 221,542 - GFF2698 0.82 -0.6 221,542 - GFF2698 0.82 -1.3 221,542 - GFF2698 0.82 +0.4 221,609 + GFF2698 0.85 +0.7 221,611 + GFF2698 0.85 -0.5 221,611 + GFF2698 0.85 +0.1 221,612 - GFF2698 0.85 -0.5 221,612 - GFF2698 0.85 -1.1 221,612 - GFF2698 0.85 -1.1 221,612 - GFF2698 0.85 -2.7 221,646 + GFF2698 0.87 -1.2 221,688 + GFF2698 0.89 -0.5 221,787 + +1.3 221,787 + -1.1 222,056 + +0.2 222,062 + +0.4 222,104 + +0.0 222,156 - -0.5 222,241 + -0.2 222,242 - +0.0 222,287 + +0.7 222,396 + GFF2699 0.12 +0.8 222,396 + GFF2699 0.12 +0.1 222,500 + GFF2699 0.18 -0.5 222,664 + GFF2699 0.26 +0.7
Or see this region's nucleotide sequence