Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2563 and GFF2564 are separated by 83 nucleotides GFF2564 and GFF2565 are separated by 24 nucleotides GFF2565 and GFF2566 are separated by 117 nucleotides
GFF2563 - Outer membrane porin protein NmpC precursor, at 80,790 to 81,878
GFF2563
GFF2564 - Gfa-like protein, at 81,962 to 82,402
GFF2564
GFF2565 - Methyl viologen resistance protein smvA, at 82,427 to 83,914
GFF2565
GFF2566 - Transcriptional regulator, TetR family, at 84,032 to 84,610
GFF2566
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-1
0
1
2 at 81.429 kb on - strand, within GFF2563 at 81.669 kb on + strand, within GFF2563 at 81.751 kb on - strand, within GFF2563 at 81.956 kb on + strand at 82.085 kb on + strand, within GFF2564 at 82.086 kb on - strand, within GFF2564 at 82.139 kb on + strand, within GFF2564 at 82.210 kb on + strand, within GFF2564 at 82.232 kb on - strand, within GFF2564 at 82.423 kb on + strand at 82.551 kb on - strand at 82.579 kb on + strand, within GFF2565 at 82.728 kb on + strand, within GFF2565 at 83.150 kb on + strand, within GFF2565 at 83.450 kb on + strand, within GFF2565 at 83.453 kb on + strand, within GFF2565 at 83.457 kb on - strand, within GFF2565 at 83.771 kb on - strand at 83.870 kb on - strand at 83.871 kb on + strand at 83.885 kb on - strand at 83.892 kb on - strand at 84.038 kb on + strand at 84.384 kb on - strand, within GFF2566 at 84.384 kb on - strand, within GFF2566 at 84.523 kb on + strand, within GFF2566 at 84.523 kb on + strand, within GFF2566 at 84.524 kb on - strand, within GFF2566 at 84.527 kb on + strand, within GFF2566 at 84.527 kb on + strand, within GFF2566 at 84.528 kb on - strand, within GFF2566 at 84.531 kb on + strand, within GFF2566 at 84.804 kb on + strand at 84.804 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 81,429 - GFF2563 0.59 -1.2 81,669 + GFF2563 0.81 +0.2 81,751 - GFF2563 0.88 -1.0 81,956 + -0.8 82,085 + GFF2564 0.28 +0.6 82,086 - GFF2564 0.28 -1.4 82,139 + GFF2564 0.40 -0.4 82,210 + GFF2564 0.56 +0.7 82,232 - GFF2564 0.61 -0.2 82,423 + -0.2 82,551 - +2.2 82,579 + GFF2565 0.10 +0.0 82,728 + GFF2565 0.20 +0.1 83,150 + GFF2565 0.49 -0.6 83,450 + GFF2565 0.69 -1.1 83,453 + GFF2565 0.69 -0.9 83,457 - GFF2565 0.69 -0.7 83,771 - -0.6 83,870 - +0.0 83,871 + -0.4 83,885 - -0.1 83,892 - +0.6 84,038 + -0.9 84,384 - GFF2566 0.61 -1.3 84,384 - GFF2566 0.61 +0.4 84,523 + GFF2566 0.85 +0.1 84,523 + GFF2566 0.85 -0.6 84,524 - GFF2566 0.85 -1.0 84,527 + GFF2566 0.85 -0.4 84,527 + GFF2566 0.85 -0.6 84,528 - GFF2566 0.86 -0.1 84,531 + GFF2566 0.86 -0.8 84,804 + -0.2 84,804 + +1.0
Or see this region's nucleotide sequence