Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2547 and GFF2548 are separated by 112 nucleotides GFF2548 and GFF2549 are separated by 56 nucleotides
GFF2547 - Aspartate aminotransferase (EC 2.6.1.1), at 60,362 to 61,564
GFF2547
GFF2548 - Glycogen debranching enzyme (EC 3.2.1.-), at 61,677 to 63,752
GFF2548
GFF2549 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 63,809 to 66,337
GFF2549
Position (kb) 61
62
63
64 Strain fitness (log2 ratio) -1
0
1 at 60.709 kb on - strand, within GFF2547 at 60.719 kb on + strand, within GFF2547 at 60.719 kb on + strand, within GFF2547 at 60.880 kb on + strand, within GFF2547 at 60.881 kb on - strand, within GFF2547 at 60.945 kb on - strand, within GFF2547 at 61.250 kb on + strand, within GFF2547 at 61.335 kb on - strand, within GFF2547 at 61.596 kb on - strand at 61.676 kb on - strand at 61.822 kb on - strand at 61.901 kb on + strand, within GFF2548 at 61.901 kb on + strand, within GFF2548 at 61.901 kb on + strand, within GFF2548 at 61.902 kb on - strand, within GFF2548 at 61.902 kb on - strand, within GFF2548 at 61.905 kb on + strand, within GFF2548 at 61.906 kb on - strand, within GFF2548 at 61.912 kb on - strand, within GFF2548 at 62.104 kb on - strand, within GFF2548 at 62.108 kb on - strand, within GFF2548 at 62.122 kb on + strand, within GFF2548 at 62.133 kb on - strand, within GFF2548 at 62.312 kb on + strand, within GFF2548 at 62.315 kb on + strand, within GFF2548 at 62.519 kb on - strand, within GFF2548 at 62.798 kb on + strand, within GFF2548 at 62.798 kb on + strand, within GFF2548 at 62.799 kb on - strand, within GFF2548 at 62.822 kb on - strand, within GFF2548 at 62.854 kb on + strand, within GFF2548 at 62.923 kb on + strand, within GFF2548 at 63.011 kb on - strand, within GFF2548 at 63.047 kb on - strand, within GFF2548 at 63.128 kb on + strand, within GFF2548 at 63.251 kb on + strand, within GFF2548 at 63.329 kb on + strand, within GFF2548 at 63.330 kb on - strand, within GFF2548 at 63.445 kb on + strand, within GFF2548 at 63.445 kb on + strand, within GFF2548 at 63.445 kb on + strand, within GFF2548 at 63.578 kb on - strand at 63.876 kb on - strand at 63.890 kb on - strand at 63.934 kb on + strand at 64.096 kb on + strand, within GFF2549 at 64.096 kb on + strand, within GFF2549 at 64.097 kb on - strand, within GFF2549 at 64.097 kb on - strand, within GFF2549 at 64.097 kb on - strand, within GFF2549 at 64.099 kb on - strand, within GFF2549 at 64.315 kb on + strand, within GFF2549 at 64.316 kb on - strand, within GFF2549 at 64.316 kb on - strand, within GFF2549 at 64.750 kb on + strand, within GFF2549
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 60,709 - GFF2547 0.29 -0.5 60,719 + GFF2547 0.30 +0.5 60,719 + GFF2547 0.30 -0.0 60,880 + GFF2547 0.43 -0.1 60,881 - GFF2547 0.43 -0.1 60,945 - GFF2547 0.48 -1.4 61,250 + GFF2547 0.74 +0.8 61,335 - GFF2547 0.81 +0.4 61,596 - -0.8 61,676 - +0.2 61,822 - -0.3 61,901 + GFF2548 0.11 +0.7 61,901 + GFF2548 0.11 -1.2 61,901 + GFF2548 0.11 -0.2 61,902 - GFF2548 0.11 -0.1 61,902 - GFF2548 0.11 -0.8 61,905 + GFF2548 0.11 -0.2 61,906 - GFF2548 0.11 +0.7 61,912 - GFF2548 0.11 -1.4 62,104 - GFF2548 0.21 -1.4 62,108 - GFF2548 0.21 -0.7 62,122 + GFF2548 0.21 -0.1 62,133 - GFF2548 0.22 -0.1 62,312 + GFF2548 0.31 -0.3 62,315 + GFF2548 0.31 -0.6 62,519 - GFF2548 0.41 -1.1 62,798 + GFF2548 0.54 -0.1 62,798 + GFF2548 0.54 +0.6 62,799 - GFF2548 0.54 -1.6 62,822 - GFF2548 0.55 +0.2 62,854 + GFF2548 0.57 -0.2 62,923 + GFF2548 0.60 -1.5 63,011 - GFF2548 0.64 +0.8 63,047 - GFF2548 0.66 +0.1 63,128 + GFF2548 0.70 -0.8 63,251 + GFF2548 0.76 +0.3 63,329 + GFF2548 0.80 +0.3 63,330 - GFF2548 0.80 -0.1 63,445 + GFF2548 0.85 -1.6 63,445 + GFF2548 0.85 +0.8 63,445 + GFF2548 0.85 -0.8 63,578 - -0.4 63,876 - -0.1 63,890 - +0.4 63,934 + +0.5 64,096 + GFF2549 0.11 -1.1 64,096 + GFF2549 0.11 +1.3 64,097 - GFF2549 0.11 +1.2 64,097 - GFF2549 0.11 -0.4 64,097 - GFF2549 0.11 -0.2 64,099 - GFF2549 0.11 -0.8 64,315 + GFF2549 0.20 +0.1 64,316 - GFF2549 0.20 -0.2 64,316 - GFF2549 0.20 +0.4 64,750 + GFF2549 0.37 -0.2
Or see this region's nucleotide sequence