Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF248 and GFF249 are separated by 63 nucleotides GFF249 and GFF250 are separated by 39 nucleotides GFF250 and GFF251 are separated by 110 nucleotides
GFF248 - ATP-dependent DNA helicase RecQ, at 21,392 to 23,239
GFF248
GFF249 - L-lysine permease, at 23,303 to 23,923
GFF249
GFF250 - Homoserine/homoserine lactone efflux protein, at 23,963 to 24,583
GFF250
GFF251 - Lysophospholipase L2 (EC 3.1.1.5), at 24,694 to 25,710
GFF251
Position (kb) 23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 22.309 kb on - strand, within GFF248 at 22.318 kb on + strand, within GFF248 at 22.319 kb on - strand, within GFF248 at 22.422 kb on + strand, within GFF248 at 22.423 kb on - strand, within GFF248 at 22.452 kb on + strand, within GFF248 at 22.560 kb on + strand, within GFF248 at 22.567 kb on + strand, within GFF248 at 22.568 kb on - strand, within GFF248 at 22.571 kb on + strand, within GFF248 at 22.571 kb on + strand, within GFF248 at 22.648 kb on - strand, within GFF248 at 22.648 kb on - strand, within GFF248 at 22.710 kb on - strand, within GFF248 at 22.781 kb on + strand, within GFF248 at 22.782 kb on - strand, within GFF248 at 23.000 kb on + strand, within GFF248 at 23.001 kb on - strand, within GFF248 at 23.313 kb on + strand at 23.314 kb on - strand at 23.767 kb on - strand, within GFF249 at 23.911 kb on + strand at 23.912 kb on - strand at 23.961 kb on + strand at 23.997 kb on - strand at 24.056 kb on - strand, within GFF250 at 24.109 kb on + strand, within GFF250 at 24.110 kb on - strand, within GFF250 at 24.145 kb on - strand, within GFF250 at 24.208 kb on + strand, within GFF250 at 24.209 kb on - strand, within GFF250 at 24.224 kb on + strand, within GFF250 at 24.335 kb on - strand, within GFF250 at 24.340 kb on - strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.425 kb on - strand, within GFF250 at 24.429 kb on - strand, within GFF250 at 24.554 kb on + strand at 24.554 kb on + strand at 24.554 kb on + strand at 24.555 kb on - strand at 24.619 kb on + strand at 24.620 kb on - strand at 24.667 kb on + strand at 24.892 kb on + strand, within GFF251 at 24.897 kb on - strand, within GFF251
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 22,309 - GFF248 0.50 +0.2 22,318 + GFF248 0.50 -3.4 22,319 - GFF248 0.50 -0.1 22,422 + GFF248 0.56 -0.5 22,423 - GFF248 0.56 +0.2 22,452 + GFF248 0.57 +1.7 22,560 + GFF248 0.63 +0.4 22,567 + GFF248 0.64 -0.0 22,568 - GFF248 0.64 +0.5 22,571 + GFF248 0.64 -0.6 22,571 + GFF248 0.64 -0.2 22,648 - GFF248 0.68 -0.4 22,648 - GFF248 0.68 +0.1 22,710 - GFF248 0.71 +2.1 22,781 + GFF248 0.75 +0.1 22,782 - GFF248 0.75 -0.3 23,000 + GFF248 0.87 +0.1 23,001 - GFF248 0.87 -0.3 23,313 + -0.0 23,314 - -0.2 23,767 - GFF249 0.75 -0.8 23,911 + -0.3 23,912 - -0.6 23,961 + +1.4 23,997 - +0.2 24,056 - GFF250 0.15 +0.6 24,109 + GFF250 0.24 +0.9 24,110 - GFF250 0.24 +0.2 24,145 - GFF250 0.29 -0.1 24,208 + GFF250 0.39 +0.4 24,209 - GFF250 0.40 +0.6 24,224 + GFF250 0.42 -0.0 24,335 - GFF250 0.60 -0.8 24,340 - GFF250 0.61 +0.8 24,424 + GFF250 0.74 -0.3 24,424 + GFF250 0.74 -0.1 24,424 + GFF250 0.74 +0.1 24,424 + GFF250 0.74 +0.5 24,425 - GFF250 0.74 +1.9 24,429 - GFF250 0.75 -0.3 24,554 + -0.0 24,554 + +0.1 24,554 + +0.1 24,555 - -0.2 24,619 + +1.0 24,620 - +0.3 24,667 + -0.1 24,892 + GFF251 0.19 +0.2 24,897 - GFF251 0.20 +0.1
Or see this region's nucleotide sequence