Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2428 and GFF2429 are separated by 246 nucleotides GFF2429 and GFF2430 are separated by 11 nucleotides GFF2430 and GFF2431 are separated by 1 nucleotides GFF2431 and GFF2432 are separated by 12 nucleotides GFF2432 and GFF2433 are separated by 195 nucleotides
GFF2428 - Peptidase B (EC 3.4.11.23), at 44,602 to 45,885
GFF2428
GFF2429 - Believed to be involved in assembly of Fe-S clusters, at 46,132 to 46,332
GFF2429
GFF2430 - Ferredoxin, 2Fe-2S, at 46,344 to 46,679
GFF2430
GFF2431 - Chaperone protein HscA, at 46,681 to 48,531
GFF2431
GFF2432 - Chaperone protein HscB, at 48,544 to 49,059
GFF2432
GFF2433 - Iron binding protein IscA for iron-sulfur cluster assembly, at 49,255 to 49,578
GFF2433
Position (kb) 46
47
48
49 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 45.793 kb on - strand at 45.793 kb on - strand at 45.815 kb on + strand at 45.944 kb on - strand at 46.063 kb on + strand at 46.071 kb on + strand at 46.071 kb on + strand at 46.071 kb on + strand at 46.287 kb on - strand, within GFF2429 at 46.332 kb on + strand at 46.336 kb on - strand at 46.516 kb on - strand, within GFF2430 at 46.769 kb on - strand at 46.951 kb on - strand, within GFF2431 at 47.631 kb on + strand, within GFF2431 at 47.788 kb on - strand, within GFF2431 at 48.210 kb on + strand, within GFF2431 at 48.448 kb on - strand at 48.535 kb on - strand at 48.808 kb on - strand, within GFF2432 at 48.808 kb on - strand, within GFF2432 at 49.121 kb on + strand at 49.122 kb on - strand at 49.237 kb on + strand at 49.257 kb on - strand at 49.460 kb on + strand, within GFF2433 at 49.460 kb on + strand, within GFF2433 at 49.460 kb on + strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 45,793 - +0.2 45,793 - +0.6 45,815 + -1.0 45,944 - +0.8 46,063 + +0.1 46,071 + +0.2 46,071 + -0.2 46,071 + +0.3 46,287 - GFF2429 0.77 +0.1 46,332 + +0.3 46,336 - -1.0 46,516 - GFF2430 0.51 +0.1 46,769 - +0.1 46,951 - GFF2431 0.15 -0.9 47,631 + GFF2431 0.51 -1.0 47,788 - GFF2431 0.60 -0.9 48,210 + GFF2431 0.83 -0.3 48,448 - +0.3 48,535 - -0.0 48,808 - GFF2432 0.51 -0.9 48,808 - GFF2432 0.51 -2.8 49,121 + -0.6 49,122 - +0.4 49,237 + -0.7 49,257 - -0.2 49,460 + GFF2433 0.63 -0.3 49,460 + GFF2433 0.63 +1.1 49,460 + GFF2433 0.63 -0.1 49,461 - GFF2433 0.64 +0.4 49,461 - GFF2433 0.64 -0.2 49,461 - GFF2433 0.64 +0.8 49,461 - GFF2433 0.64 -0.8 49,461 - GFF2433 0.64 -2.8
Or see this region's nucleotide sequence