Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2392

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2391 and GFF2392 are separated by 15 nucleotidesGFF2392 and GFF2393 are separated by 131 nucleotides GFF2391 - Methionyl-tRNA formyltransferase (EC 2.1.2.9), at 19,956 to 20,903 GFF2391 GFF2392 - Peptide deformylase (EC 3.5.1.88), at 20,919 to 21,428 GFF2392 GFF2393 - Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake, at 21,560 to 22,684 GFF2393 Position (kb) 20 21 22Strain fitness (log2 ratio) -1 0 1at 20.993 kb on - strand, within GFF2392at 21.593 kb on + strandat 21.643 kb on + strandat 21.750 kb on + strand, within GFF2393at 21.803 kb on + strand, within GFF2393at 21.803 kb on + strand, within GFF2393at 21.804 kb on - strand, within GFF2393at 21.877 kb on + strand, within GFF2393at 21.920 kb on + strand, within GFF2393at 22.324 kb on + strand, within GFF2393at 22.413 kb on - strand, within GFF2393at 22.420 kb on - strand, within GFF2393

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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20,993 - GFF2392 0.15 -1.1
21,593 + -0.1
21,643 + +0.7
21,750 + GFF2393 0.17 +0.8
21,803 + GFF2393 0.22 -0.4
21,803 + GFF2393 0.22 -0.1
21,804 - GFF2393 0.22 -0.1
21,877 + GFF2393 0.28 +0.3
21,920 + GFF2393 0.32 -0.0
22,324 + GFF2393 0.68 +0.8
22,413 - GFF2393 0.76 -1.7
22,420 - GFF2393 0.76 +0.2

Or see this region's nucleotide sequence