Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF236

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF234 and GFF235 are separated by 258 nucleotidesGFF235 and GFF236 are separated by 185 nucleotidesGFF236 and GFF237 are separated by 36 nucleotidesGFF237 and GFF238 overlap by 4 nucleotides GFF234 - STMD1.44 protein, at 9,771 to 10,160 GFF234 GFF235 - possible exported protein, at 10,419 to 10,670 GFF235 GFF236 - FIG00545237: hypothetical protein, at 10,856 to 11,059 GFF236 GFF237 - Diaminopimelate epimerase (EC 5.1.1.7), at 11,096 to 11,920 GFF237 GFF238 - Protein of unknown function DUF484, at 11,917 to 12,624 GFF238 Position (kb) 10 11 12Strain fitness (log2 ratio) -3 -2 -1 0 1at 10.002 kb on + strand, within GFF234at 10.049 kb on + strand, within GFF234at 10.062 kb on - strand, within GFF234at 10.069 kb on + strand, within GFF234at 10.070 kb on - strand, within GFF234at 10.118 kb on + strand, within GFF234at 10.192 kb on + strandat 10.204 kb on - strandat 10.225 kb on + strandat 10.290 kb on - strandat 10.549 kb on + strand, within GFF235at 10.670 kb on + strandat 10.670 kb on + strandat 10.689 kb on + strandat 10.689 kb on + strandat 10.690 kb on - strandat 10.690 kb on - strandat 10.693 kb on + strandat 10.742 kb on - strandat 10.774 kb on + strandat 10.841 kb on + strandat 10.841 kb on + strandat 10.870 kb on + strandat 10.935 kb on - strand, within GFF236at 11.054 kb on + strandat 11.917 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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10,002 + GFF234 0.59 +1.3
10,049 + GFF234 0.71 -0.5
10,062 - GFF234 0.75 -0.5
10,069 + GFF234 0.76 -0.8
10,070 - GFF234 0.77 +1.0
10,118 + GFF234 0.89 +0.5
10,192 + +0.1
10,204 - -1.6
10,225 + -1.8
10,290 - +0.2
10,549 + GFF235 0.52 +1.1
10,670 + +0.4
10,670 + +1.1
10,689 + +0.2
10,689 + +0.3
10,690 - +0.0
10,690 - +0.1
10,693 + +0.8
10,742 - -0.4
10,774 + -0.9
10,841 + +0.2
10,841 + +0.0
10,870 + -0.8
10,935 - GFF236 0.39 -1.1
11,054 + +0.4
11,917 - -3.0

Or see this region's nucleotide sequence